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Literature summary for 2.7.1.25 extracted from

  • Lansdon, E.B.; Segel, I.H.; Fisher, A.J.
    Ligand-induced structural changes in adenosine 5'-phosphosulfate kinase from Penicillium chrysogenum (2002), Biochemistry, 41, 13672-13680.
    View publication on PubMed
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Crystallization (Commentary)

Crystallization (Comment) Organism
crystals are grown in 1.7 M NaH2PO4, 300 mM K2HPO4 and 100 mM Na-succinate, pH 4.0 by hanging drop vapor diffusion at room temperature, crystal structure of E-ADP-APS ternary complex at 1.43 A, crystal structure of E-ADP binary complex at 2.0 A Penicillium chrysogenum

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-phosphosulfate adenosine 5'-phosphosulfate can bind to E-MgADP forming a catalytically inactive E-MgADP-APS ternary complex Penicillium chrysogenum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23670
-
 2 * 23670 Penicillium chrysogenum

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum Q12657
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate enzyme follows a compulsory ordered mechanism in which MgATP2- binds before APS, and PAPS leaves before MgADP- Penicillium chrysogenum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Penicillium chrysogenum ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?

Subunits

Subunits Comment Organism
dimer 2 * 23670 Penicillium chrysogenum