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Literature summary for 2.7.1.25 extracted from

  • Lillig, C.H.; Schiffmann, S.; Berndt, C.; Berken, A.; Tischka, R.; Schwenn, J.D.
    Molecular and catalytic properties of Arabidopsis thaliana adenylyl sulfate (APS)-kinase (2001), Arch. Biochem. Biophys., 392, 303-310.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activity declines rapidly in the absence of dithiothreitol Arabidopsis thaliana
high ionic strength maximal activity at a high concentration of buffer-salts either alone or plus dithiothreitol and thioredoxin 1 from Escherichia coli Arabidopsis thaliana
thioredoxin essential for activity Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
S182C similar catalytic activity as wild-type Arabidopsis thaliana
S182F similar catalytic activity as wild-type Arabidopsis thaliana

General Stability

General Stability Organism
recombinant enzyme is rapidly inactivated by dialysis, dilution or freezing even in the presence of glycerol Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-phosphosulfate uncompetitive inhibition above 0.001 mM Arabidopsis thaliana
EDTA complete inhibition Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00014
-
adenosine 5'-phosphosulfate pH 7.4, 25°C Arabidopsis thaliana
0.147
-
ATP pH 7.4, 25°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31977
-
x * 31977, deduced from nucleotide sequence Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O49196 APS kinase gene Atakn1
-
Arabidopsis thaliana O49196 APS kinase gene Atakn2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Arabidopsis thaliana ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?

Subunits

Subunits Comment Organism
? x * 31977, deduced from nucleotide sequence Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0045
-
adenosine 5'-phosphosulfate pH 7.4, 25°C Arabidopsis thaliana