Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | activity declines rapidly in the absence of dithiothreitol | Arabidopsis thaliana | |
high ionic strength | maximal activity at a high concentration of buffer-salts either alone or plus dithiothreitol and thioredoxin 1 from Escherichia coli | Arabidopsis thaliana | |
thioredoxin | essential for activity | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
S182C | similar catalytic activity as wild-type | Arabidopsis thaliana |
S182F | similar catalytic activity as wild-type | Arabidopsis thaliana |
General Stability | Organism |
---|---|
recombinant enzyme is rapidly inactivated by dialysis, dilution or freezing even in the presence of glycerol | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-phosphosulfate | uncompetitive inhibition above 0.001 mM | Arabidopsis thaliana | |
EDTA | complete inhibition | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00014 | - |
adenosine 5'-phosphosulfate | pH 7.4, 25°C | Arabidopsis thaliana | |
0.147 | - |
ATP | pH 7.4, 25°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31977 | - |
x * 31977, deduced from nucleotide sequence | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O49196 | APS kinase gene Atakn1 | - |
Arabidopsis thaliana | O49196 | APS kinase gene Atakn2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Arabidopsis thaliana | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31977, deduced from nucleotide sequence | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
adenosine 5'-phosphosulfate | pH 7.4, 25°C | Arabidopsis thaliana |