Cloned (Comment) | Organism |
---|---|
expression of full-length PAPS synthase and 1-268 N-terminal fragment in COS-1 cells and Escherichia coli | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
adenosine 5'-phosphosulfate | pH 8.0, 37°C, COS-1 cell-expressed full length PAPS synthase | Homo sapiens | |
0.0006 | - |
adenosine 5'-phosphosulfate | pH 8.0, 37°C, COS-1 cell-expressed N-terminal fragment | Homo sapiens | |
0.0023 | - |
adenosine 5'-phosphosulfate | pH 8.0, 37°C, Escherichia coli-expressed N-terminal fragment | Homo sapiens | |
0.0026 | - |
adenosine 5'-phosphosulfate | pH 8.0, 37°C, Escherichia coli-expressed full length PAPS synthase | Homo sapiens | |
0.23 | - |
ATP | pH 8.0, 37°C, COS-1 cell-expressed N-terminal fragment | Homo sapiens | |
0.26 | - |
ATP | pH 8.0, 37°C, E. coli expressed N-terminal fragment | Homo sapiens | |
0.45 | - |
ATP | pH 8.0, 37°C, E. coli-expressed full length PAPS synthase | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O43252 | bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5-phosphosulfate | enzyme has both ATP sulfurylase and APS kinase activity | Homo sapiens | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | ? | |
ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Homo sapiens | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | ? |
Synonyms | Comment | Organism |
---|---|---|
PAPS synthase | - |
- |