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Literature summary for 2.7.1.24 extracted from
- Identification of yacE (coaE) as the structural gene for dephosphocoenzyme A kinase in Escherichia coli K-12 (2001), J. Bacteriol., 183, 2774-2778.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene coaE, DNA sequence determination and analysis, overexpression in Escherichia coli strain W3110 | Corynebacterium ammoniagenes |
| gene yacE, DNA sequence analysis | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
ATP | native wild-type enzyme, pH 8.5, 25°C | Corynebacterium ammoniagenes |  |
| 0.76 | - |
dephospho-CoA | native wild-type enzyme, pH 8.5, 25°C | Corynebacterium ammoniagenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22200 | - |
recombinant enzyme, gel filtration | Corynebacterium ammoniagenes |
| 22600 | - |
1 * 22600, deduced from DNA sequence | Escherichia coli |
| 25000 | - |
native enzyme, gel filtration | Corynebacterium ammoniagenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dephospho-CoA | Corynebacterium ammoniagenes | last step of coenzyme A biosynthesis: phosphorylation of the 3'-OH group of the ribose sugar moiety | ADP + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium ammoniagenes | - |
gene coaE | - |
| Escherichia coli | P0A6I9 | gene caoE, formerly yacE | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native wild-type enzyme, 2800fold to homogeneity | Corynebacterium ammoniagenes |
| recombinant wild-type from Escherichia coli W3110, 22fold | Corynebacterium ammoniagenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
purified native wild-type enzyme | Corynebacterium ammoniagenes |
| 22 | - |
purified recombinant wild-type enzyme | Corynebacterium ammoniagenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dephospho-CoA | - |
Corynebacterium ammoniagenes | ADP + CoA | - |
ir | |
| ATP + dephospho-CoA | - |
Escherichia coli | ADP + CoA | - |
ir | |
| ATP + dephospho-CoA | last step of coenzyme A biosynthesis: phosphorylation of the 3'-OH group of the ribose sugar moiety | Corynebacterium ammoniagenes | ADP + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 1 * 22600, deduced from DNA sequence | Escherichia coli |
| monomer | 1 * 22200, recombinant wild-type enzyme, SDS-PAGE | Corynebacterium ammoniagenes |
| monomer | 1 * 25000, native wild-type enzyme, SDS-PAGE | Corynebacterium ammoniagenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Corynebacterium ammoniagenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Escherichia coli |
| 8.5 | - |
recombinant enzyme, broad | Corynebacterium ammoniagenes |
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