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Literature summary for 2.7.1.232 extracted from
- Identification, characterization of levoglucosan kinase, and cloning and expression of levoglucosan kinase cDNA from Aspergillus niger CBX-209 in Escherichia coli (2002), Protein Expr. Purif., 1, 71-81.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene lgk, recombinant expression in Escherichia coli strain DH5alpha | Aspergillus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | competitive inhibition | Aspergillus niger |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
ATP | pH 9.3, 30°C | Aspergillus niger | |
| 71.2 | - |
1,6-anhydro-beta-D-glucopyranose | pH 9.3, 30°C | Aspergillus niger | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 56000, SDS-PAGE | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + levoglucosan + H2O | Aspergillus niger | - |
ADP + D-glucopyranose 6-phosphate | - |
? | |
| ATP + levoglucosan + H2O | Aspergillus niger CBX-209 | - |
ADP + D-glucopyranose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
gene lgk | - |
| Aspergillus niger CBX-209 | - |
gene lgk | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme to apparent homogeneity by using a combination of seven purification steps | Aspergillus niger |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, partly purified enzyme, stable at | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + levoglucosan + H2O | - |
Aspergillus niger | ADP + D-glucopyranose 6-phosphate | - |
? | |
| ATP + levoglucosan + H2O | - |
Aspergillus niger | ADP + D-glucopyranose 6-phosphate | FAB-mass spectrometric method for product identification | ? | |
| ATP + levoglucosan + H2O | - |
Aspergillus niger CBX-209 | ADP + D-glucopyranose 6-phosphate | - |
? | |
| ATP + levoglucosan + H2O | - |
Aspergillus niger CBX-209 | ADP + D-glucopyranose 6-phosphate | FAB-mass spectrometric method for product identification | ? | |
| additional information | substrate specificity for levoglucosan, not only the structure of the intramolecular glucosidic linkage but also the configuration of the pyranose frame is specific for recognition by levoglucosan kinase | Aspergillus niger | ? | - |
? | |
| additional information | substrate specificity for levoglucosan, not only the structure of the intramolecular glucosidic linkage but also the configuration of the pyranose frame is specific for recognition by levoglucosan kinase | Aspergillus niger CBX-209 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, SDS-PAGE | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LGK | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
levoglucosan can protect the enzyme from thermal inactivation | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.3 | - |
- |
Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | partly purified enzyme, stable at | Aspergillus niger |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Aspergillus niger | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
ADP | pH 9.3, 30°C | Aspergillus niger | |
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