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Literature summary for 2.7.1.23 extracted from

  • Prasad, U.V.; Vasu, D.; Gowtham, R.R.; Pradeep, C.K.; Swarupa, V.; Yeswanth, S.; Choudhary, A.; Sarma, P.V.G.K.
    Cloning, expression and characterization of NAD kinase from Staphylococcus aureus involved in the formation of NADP(H) a key molecule in the maintaining of redox status and biofilm formation (2017), Adv. Biomed. Res., 6, 97 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.64
-
 ATP wild-type enzyme, pH and temperature not specified in the publication Staphylococcus aureus
0.66
-
 ATP recombinant enzyme, pH and temperature not specified in the publication Staphylococcus aureus
0.99
-
 NADP+ recombinant enzyme, pH and temperature not specified in the publication Staphylococcus aureus
1.01
-
 NADP+ wild-type enzyme, pH and temperature not specified in the publication Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Staphylococcus aureus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 SDS-PAGE Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + NADP+ Staphylococcus aureus
-
 ATP + NAD+
-
 ?
ADP + NADP+ Staphylococcus aureus ATCC 12600
-
 ATP + NAD+
-
 ?
ATP + NAD+ Staphylococcus aureus
-
 ADP + NADP+
-
 ?
ATP + NAD+ Staphylococcus aureus ATCC 12600
-
 ADP + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus H6UH60
-
-
Staphylococcus aureus ATCC 12600 H6UH60
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + NADP+
-
 Staphylococcus aureus ATP + NAD+
-
 ?
ADP + NADP+
-
 Staphylococcus aureus ATCC 12600 ATP + NAD+
-
 ?
ATP + NAD+
-
 Staphylococcus aureus ADP + NADP+
-
 ?
ATP + NAD+ the enzyme exhibits very high affinity for NAD+ compared to adenosine triphosphate Staphylococcus aureus ADP + NADP+
-
 ?
ATP + NAD+
-
 Staphylococcus aureus ATCC 12600 ADP + NADP+
-
 ?
ATP + NAD+ the enzyme exhibits very high affinity for NAD+ compared to adenosine triphosphate Staphylococcus aureus ATCC 12600 ADP + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Staphylococcus aureus

Synonyms

Synonyms Comment Organism
NADK
-
 Staphylococcus aureus

General Information

General Information Comment Organism
physiological function the enzyme is involved in the formation of NADP(H). It is a key molecule in the maintaining of redox status and biofilm formation Staphylococcus aureus