Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Listeria monocytogenes |
Crystallization (Comment) | Organism |
---|---|
enzyme bound to compounds 5'-O-propargyl-5'-deoxyadenosine, 5'-azido-5'-deoxyadenosine, 8-bromo-5'-amino-5'-deoxyadenosine, or 8-thioglycolic-5'-azido-5'-deoxyadenosine, using 30 mM sodium bromide, 220 mM potassium citrate, pH 4.8-5.1, 6% glycerol, 15%-16% (w/v) polyethylene glycol 400 | Listeria monocytogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-([6-amino-9-[(2R,3R,4S,5R)-5-(aminomethyl)-3,4-dihydroxytetrahydrofuran-2-yl]-9H-purin-8-yl]sulfanyl)-N-[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]acetamide | - |
Listeria monocytogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Listeria monocytogenes | Q8Y8D7 | - |
- |
Purification (Comment) | Organism |
---|---|
cobalt-based IMAC resin column chromatography | Listeria monocytogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAD+ | - |
Listeria monocytogenes | ADP + NADP+ | - |
? | |
additional information | the NAD kinase from Listeria monocytogenes can promote amide formation between 5'-amino-5'-deoxyadenosine and carboxylic acid groups | Listeria monocytogenes | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD kinase | - |
Listeria monocytogenes |
NADK1 | - |
Listeria monocytogenes |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
2-([6-amino-9-[(2R,3R,4S,5R)-5-(aminomethyl)-3,4-dihydroxytetrahydrofuran-2-yl]-9H-purin-8-yl]sulfanyl)-N-[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]acetamide | in 50 mM Tris-HCl, pH 7.4, 10 mM MgCl2, at 30°C | Listeria monocytogenes |