Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta-gami(DE3)pLysS cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by NADP+ | Homo sapiens | |
NADH | 40% residual activity at 0.5 mM | Homo sapiens | |
NADPH | 66% residual activity at 0.5 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.07 | - |
NAD+ | at pH 8.0 and 50°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
4 * 43000, SDS-PAGE | Homo sapiens |
172000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
TALON column chromatography and Mono Q column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAD+ | - |
Homo sapiens | ADP + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 43000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
NAD kinase | - |
Homo sapiens |
NADK | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Homo sapiens |