Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
crystals of SeMet-containing PPNK_THEMA are obtained at room temperature in hanging drops. Crystal structure of inorganic polyphosphate/ATP-NAD kinase is determined at 2.3 A resolution. The crystal structure is solved using single-wavelength anomalous diffraction data collected at the Se absorption-peak wavelength in a state in which no cofactors or substrates are bound. It revealed that the 258-amino-acid protein is folded into two distinct domains. The N-terminal alpha/beta-domain spans the first 100 amino acids and the last 30 amino acids of the polypeptide and has several topological matches in the PDB, whereas the other domain, which spans the middle 130 residues, adopts a unique beta-sandwich architecture and only appreciably matches the recently deposited PDB structures of NAD kinases | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAD+ | Thermotoga maritima | - |
ADP + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X255 | - |
- |
Renatured (Comment) | Organism |
---|---|
recombinant enzyme | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAD+ | - |
Thermotoga maritima | ADP + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PPNK_THEMA | - |
Thermotoga maritima |