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Literature summary for 2.7.1.23 extracted from

  • Mori, S.; Kawai, S.; Shi, F.; Mikami, B.; Murata, K.
    Molecular conversion of NAD Kinase to NADH kinase through single amino acid residue substitution (2005), J. Biol. Chem., 280, 24104-24112.
    View publication on PubMed
Search for more literature for 2.7.1.23

Protein Variants

Protein Variants Comment Organism
G183R decrease both in NAD+ kinase and NADH kinase activity Micrococcus flavus
R175E no conversion of NAD+ kinase activity to NADH kinase activity Escherichia coli
R175G shows both ATP-dependent NAD+ kinase activity and NADH kinase activity Escherichia coli
R175H shows both ATP-dependent NAD+ kinase activity and NADH kinase activity Escherichia coli
R175I no conversion of NAD+ kinase activity to NADH kinase activity Escherichia coli
R175K no conversion of NAD+ kinase activity to NADH kinase activity Escherichia coli
R175Q shows both ATP-dependent NAD+ kinase activity and NADH kinase activity Escherichia coli
R175T shows both ATP-dependent NAD+ kinase activity and NADH kinase activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 ATP pH 7.0, 37°C, mutant G183R Micrococcus flavus
0.2
-
 ATP pH 7.0, 37°C, mutant R175G Escherichia coli
0.21
-
 poly(P)4 pH 7.0, 37°C, mutant G183R Micrococcus flavus
0.23
-
 ATP pH 7.0, 37°C, wild-type Escherichia coli
0.23
-
 ATP pH 7.0, 37°C, wild-type Micrococcus flavus
0.26
-
 NAD+ pH 7.0, 37°C, cosubstrate poly(P)4, wild-type Micrococcus flavus
0.33
-
 poly(P)4 pH 7.0, 37°C, wild-type Micrococcus flavus
0.53
-
 NAD+ pH 7.0, 37°C, cosubstrate ATP, wild-type Micrococcus flavus
1.43
-
 NAD+ pH 7.0, 37°C, cosubstrate ATP, mutant G183R Micrococcus flavus
1.85
-
 NAD+ pH 7.0, 37°C, wild-type Escherichia coli
1.86
-
 NAD+ pH 7.0, 37°C, cosubstrate poly(P)4, mutant G183R Micrococcus flavus
2
-
 NAD+ pH 7.0, 37°C, mutant R175G Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Micrococcus flavus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4
-
 pH 7.0, 37°C, substrate NAD+, cosubstrate poly(P)4, mutant G183R Micrococcus flavus
1.78
-
 pH 7.0, 37°C, substrate NAD+, cosubstrate ATP, mutant G183R Micrococcus flavus
2.33
-
 pH 7.0, 37°C, mutant R175G, substrate NAD+ Escherichia coli
2.68
-
 pH 7.0, 37°C, mutant R175, substrate ATP Escherichia coli
8.63
-
 pH 7.0, 37°C, substrate NAD+, cosubstrate poly(P)4, wild-type Micrococcus flavus
12.45
-
 pH 7.0, 37°C, wild-type, substrate ATP Escherichia coli
12.86
-
 pH 7.0, 37°C, wild-type, substrate NAD+ Escherichia coli
18.67
-
 pH 7.0, 37°C, substrate NAD+, cosubstrate ATP, wild-type Micrococcus flavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + ATP
-
 Escherichia coli NADP+ + ADP
-
 ?
NAD+ + ATP
-
 Micrococcus flavus NADP+ + ADP
-
 ?
NAD+ + poly(P)4
-
 Micrococcus flavus NADP+ + poly(P)3
-
 ?

Synonyms

Synonyms Comment Organism
NADK
-
 Micrococcus flavus
YfjB
-
 Escherichia coli