Literature summary for 2.7.1.23 extracted from
Gallais, S.; de Crescenzo, M.A.; Laval-Martin, D.L.
Characterization of soluble calcium calmodulin-dependent and -independent NAD+ kinases from Avena sativa seeds (2001), Aust. J. Plant Physiol., 28, 363-371.
No PubMed abstract available
Activating Compound
Activating Compound |
Comment |
Organism |
Structure |
adenine |
5 mM, activity of both isoenzymes is modestly enhanced |
Avena sativa |
|
AMP |
5 mM, activity of both isoenzymes is modestly enhanced |
Avena sativa |
|
Caffeine |
5 mM, activity of both isoenzymes is modestly enhanced |
Avena sativa |
|
Calmodulin |
a 410000 Da Ca2+-calmodulin-independent isoform and a 63000 Da Ca2+-calmodulin-dependent isoform |
Avena sativa |
|
D-ribose 5-phosphate |
5 mM, activity of both isoenzymes is modestly enhanced |
Avena sativa |
|
imidazole |
5 mM, activity of both isoenzymes is modestly enhanced |
Avena sativa |
|
nicotinic acid |
5 mM, activity of both isoenzymes is modestly enhanced |
Avena sativa |
|
Pyridine |
5 mM, activity of both isoenzymes is modestly enhanced |
Avena sativa |
|
General Stability
General Stability |
Organism |
partial loss of activity after freezing and thawing |
Avena sativa |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
ADP |
5 mM, 4% inhibition of the 410000 Da isoform, 25% inhibition of the 63000 Da isoform |
Avena sativa |
|
chlorpromazin |
5 mM, 11% inhibition of the 410000 Da isoform, 80% inhibition of the 63000 Da isoform |
Avena sativa |
|
diphosphate |
5 mM, 46% inhibition of the 410000 Da isoform, 69% inhibition of the 63000 Da isoform |
Avena sativa |
|
EGTA |
5 mM, 20% inhibition of the 410000 Da isoform, 85% inhibition of the 63000 Da isoform |
Avena sativa |
|
iodoacetic acid |
1 mM, 56% inhibition of the 63000 Da isoform, no inhibition of the 410000 Da isoform |
Avena sativa |
|
NEM |
1 mM, 92% inhibition of the 63000 Da isoform, 34% inhibition of the 410000 Da isoform |
Avena sativa |
|
R24571 |
1 mM, 46% inhibition of the 410000 Da isoform, 89% inhibition of the 63000 da isoform |
Avena sativa |
|
Trifluoperazine |
2 mM, 8% inhibition of the 410000 da isoform, 83% inhibition of the 63000 Da isoform |
Avena sativa |
|
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Ca2+ |
a 410000 Da Ca2+-calmodulin-independent isoform and a 63000 Da Ca2+-calmodulin-dependent isoform |
Avena sativa |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
63000 |
- |
Ca2+-calmodulin-dependent isoenzyme |
Avena sativa |
410000 |
- |
Ca2+-calmodulin-independent NAD+ kinase, gel filtration |
Avena sativa |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
ATP + NAD+ |
Avena sativa |
the 410000 Da isoenzyme could be a housekeeping enzyme, the 63000 Da isoenzyme could be mainly involved in the adaption and response of Avena sativa to environmental signals or stress through changes of redox potential and/or calcium signalling pathways |
ADP + NADP+ |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Avena sativa |
- |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
- |
Avena sativa |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
seed |
culture of after-ripened and dormant seeds |
Avena sativa |
- |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
0.0009966 |
- |
- |
Avena sativa |
Storage Stability
Storage Stability |
Organism |
-80°C, 30% v/v glycerol, stable for more than 3 months |
Avena sativa |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
ATP + NAD+ |
the 410000 Da isoenzyme could be a housekeeping enzyme, the 63000 Da isoenzyme could be mainly involved in the adaption and response of Avena sativa to environmental signals or stress through changes of redox potential and/or calcium signalling pathways |
Avena sativa |
ADP + NADP+ |
- |
? |
|
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
7.5 |
8 |
410000 Da Ca2+-calmodulin-independent isoform and 63000 Da Ca2+-calmodulin-dependent isoform |
Avena sativa |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
6.8 |
9.2 |
90% of maximal activity between pH 6.8 and pH 9.2, 410000 Da Ca2+-calmodulin-independent isoform |
Avena sativa |
7.2 |
8.5 |
90% of maximal activity between pH 7.2 and pH 8.5, 63000 Da Ca2+-calmodulin-dependent isoform |
Avena sativa |