Cloned (Comment) | Organism |
---|---|
cDNA is amplified and functionally overexpressed in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-acetyl-pyridine adenine dinucleotide | 1 mM, 30% inhibition | Homo sapiens | |
3-aldehyde-pyridine adenine dinucleotide | 1 mM, 32% inhibition | Homo sapiens | |
nicotinamide guanine dinucleotide | 1 mM, 23% inhibition | Homo sapiens | |
nicotinamide hypoxanthine dinucleotide | 1 mM, 24% inhibition | Homo sapiens | |
nicotinic acid adenine dinucleotide phosphate | 1 mM, 37% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
NAD+ | 30°C | Homo sapiens | |
3.3 | - |
ATP | 30°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal concentration is 10 mM. At 1 mM, 29% of the activation with 1 mM Zn2+ | Homo sapiens | |
Mn2+ | 1 mM, 83% of the activation with 1 mM Zn2+ | Homo sapiens | |
Zn2+ | 1 mM, highest activation of divalent metal ions tested | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49228 | - |
4 * 49228, calculation from nucleotide sequence | Homo sapiens |
200000 | - |
gel filtration | Bos taurus |
200000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAD+ | Homo sapiens | synthesis of NADP+ | ADP + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | O95544 | - |
- |
Purification (Comment) | Organism |
---|---|
partial | Bos taurus |
recombinant enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | - |
Homo sapiens | - |
liver | - |
Bos taurus | - |
placenta | - |
Homo sapiens | - |
skeletal muscle | no expression of NAD kinase in skeletal muscle | Homo sapiens | - |
small intestine | little expression | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.7 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAD+ | - |
Bos taurus | ADP + NADP+ | - |
? | |
ATP + NAD+ | - |
Homo sapiens | ADP + NADP+ | - |
? | |
ATP + NAD+ | synthesis of NADP+ | Homo sapiens | ADP + NADP+ | - |
? | |
GTP + NAD+ | 7% of the activity with ATP | Homo sapiens | GDP + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 4 * 49228, calculation from nucleotide sequence | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
5 min, less than 5% loss of activity | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
rapid inactivation below | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bos taurus | |
NAD+ | - |
Homo sapiens |