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Literature summary for 2.7.1.23 extracted from

  • Lerner, F.; Niere, M.; Ludwig, A.; Ziegler, M.
    Structural and functional characterization of human NAD kinase (2001), Biochem. Biophys. Res. Commun., 288, 69-74.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cDNA is amplified and functionally overexpressed in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3-acetyl-pyridine adenine dinucleotide 1 mM, 30% inhibition Homo sapiens
3-aldehyde-pyridine adenine dinucleotide 1 mM, 32% inhibition Homo sapiens
nicotinamide guanine dinucleotide 1 mM, 23% inhibition Homo sapiens
nicotinamide hypoxanthine dinucleotide 1 mM, 24% inhibition Homo sapiens
nicotinic acid adenine dinucleotide phosphate 1 mM, 37% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.54
-
NAD+ 30°C Homo sapiens
3.3
-
ATP 30°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal concentration is 10 mM. At 1 mM, 29% of the activation with 1 mM Zn2+ Homo sapiens
Mn2+ 1 mM, 83% of the activation with 1 mM Zn2+ Homo sapiens
Zn2+ 1 mM, highest activation of divalent metal ions tested Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49228
-
4 * 49228, calculation from nucleotide sequence Homo sapiens
200000
-
gel filtration Bos taurus
200000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + NAD+ Homo sapiens synthesis of NADP+ ADP + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens O95544
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Bos taurus
recombinant enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast
-
Homo sapiens
-
liver
-
Bos taurus
-
placenta
-
Homo sapiens
-
skeletal muscle no expression of NAD kinase in skeletal muscle Homo sapiens
-
small intestine little expression Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.7
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NAD+
-
Bos taurus ADP + NADP+
-
?
ATP + NAD+
-
Homo sapiens ADP + NADP+
-
?
ATP + NAD+ synthesis of NADP+ Homo sapiens ADP + NADP+
-
?
GTP + NAD+ 7% of the activity with ATP Homo sapiens GDP + NADP+
-
?

Subunits

Subunits Comment Organism
hexamer 4 * 49228, calculation from nucleotide sequence Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
5 min, less than 5% loss of activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
rapid inactivation below Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bos taurus
NAD+
-
Homo sapiens