Activating Compound | Comment | Organism | Structure |
---|---|---|---|
quinolinic acid | allosteric activator | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | 1 mM, 75% inhibition | Bacillus subtilis | |
NADP+ | 0.05 mM, 75% inhibition | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
NAD+ | - |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, SDS-PAGE | Bacillus subtilis |
60000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAD+ | Bacillus subtilis | key enzyme for NADP+ metabolism and quinolinic acid metabolism | ADP + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAD+ | - |
Bacillus subtilis | ADP + NADP+ | - |
? | |
ATP + NAD+ | key enzyme for NADP+ metabolism and quinolinic acid metabolism | Bacillus subtilis | ADP + NADP+ | - |
? | |
dATP + NAD+ | 77% of the activity with ATP | Bacillus subtilis | dADP + NADP+ | - |
? | |
dCTP + NAD+ | 30% of the activity with ATP | Bacillus subtilis | dCDP + NADP+ | - |
? | |
dGTP + NAD+ | 77% of the activity with ATP | Bacillus subtilis | dGDP + NADP+ | - |
? | |
diphosphate + NAD+ | 10% of the activity with ATP | Bacillus subtilis | phosphate + NADP+ | - |
? | |
dTTP + NAD+ | 35% of the activity with ATP | Bacillus subtilis | dTDP + NADP+ | - |
? | |
GTP + NAD+ | 69% of the activity with ATP | Bacillus subtilis | GDP + NADP+ | - |
? | |
polyphosphate + NAD+ | 50% of the activity with ATP | Bacillus subtilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | 25°C: about 45% of maximal activity, 50°C: about 70% of maximal activity | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
15 min, 50% loss of activity | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.36 | - |
ATP | - |
Bacillus subtilis | |
5.46 | - |
NAD+ | - |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
glycine-NaOH buffer | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | pH 7.0: about 35% of maximal activity, pH 10.0: about 50% of maximal activity | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus subtilis |