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Literature summary for 2.7.1.221 extracted from

  • Ukaegbu, O.I.; DeMeester, K.E.; Liang, H.; Brown, A.R.; Jones, Z.S.; Grimes, C.L.
    Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates (2020), Methods Enzymol., 638, 1-26 .
    View publication on PubMed
Search for more literature for 2.7.1.221

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0303
-
 2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida
0.0304
-
 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida
0.55
-
 2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida
1.12
-
 2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM used in assay conditions Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-D-muramate Pseudomonas putida
-
 ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography and Superdex S200 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ((2R)-2-(((3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl)oxy)propanoyl)-L-alanine
-
 Pseudomonas putida ADP + ((2R)-2-(((3R,4R,5S,6R)-3-acetamido-5-hydroxy-6-(hydroxymethyl)-2-(phosphonooxy)tetrahydro-2H-pyran-4-yl)oxy)propanoyl)-L-alanine
-
 ?
ATP + 2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
 Pseudomonas putida ADP + 2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose
-
 ?
ATP + 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
 Pseudomonas putida ADP + 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose
-
 ?
ATP + 2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
 Pseudomonas putida ADP + 2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose
-
 ?
ATP + 2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
 Pseudomonas putida ADP + 2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose
-
 ?
ATP + 2-[3-[3-(but-3-yn-1-yl)-3H-diazirin-3-yl]propanamido]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
 Pseudomonas putida ADP + 2-[3-[3-(but-3-yn-1-yl)-3H-diazirin-3-yl]propanamido]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose
-
 ?
ATP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-(2,2-difluoroacetamido)-D-glucopyranose
-
 Pseudomonas putida ADP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-(2,2-difluoroacetamido)-1-O-phosphono-D-glucopyranose
-
 ?
ATP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-(3-[[2-([5-[(3aR,4R,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl]amino)ethyl]disulfanyl]propanamido)-D-glucopyranose
-
 Pseudomonas putida ADP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-(3-[[2-([5-[(3aR,4R,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl]amino)ethyl]disulfanyl]propanamido)-1-O-phosphono-D-glucopyranose
-
 ?
ATP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-([5-[(3aR,6R,6aS)-2-oxohexahydro-1H-thieno[2,3-d]imidazol-6-yl]pentanoyl]amino)-D-glucopyranose
-
 Pseudomonas putida ADP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-([5-[(3aR,6R,6aS)-2-oxohexahydro-1H-thieno[2,3-d]imidazol-6-yl]pentanoyl]amino)-1-O-phosphono-D-glucopyranose
-
 ?
ATP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[(4-oxopentanoyl)amino]-D-glucopyranose
-
 Pseudomonas putida ADP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[(4-oxopentanoyl)amino]-1-O-phosphono-D-glucopyranose
-
 ?
ATP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[2-[4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenyl]acetamido]-D-glucopyranose
-
 Pseudomonas putida ADP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[2-[4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenyl]acetamido]-1-O-phosphono-D-glucopyranose
-
 ?
ATP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[3-(3-methyl-3H-diazirin-3-yl)propanamido]-D-glucopyranose
-
 Pseudomonas putida ADP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[3-(3-methyl-3H-diazirin-3-yl)propanamido]-1-O-phosphono-D-glucopyranose
-
 ?
ATP + N-acetyl-D-muramate
-
 Pseudomonas putida ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
amgK
-
 Pseudomonas putida
MurNAc/GlcNAc anomeric kinase
-
 Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.67
-
 2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida
6.58
-
 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida
9.74
-
 2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida
14.07
-
 2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.02
-
 2-[3-[3-(but-3-yn-1-yl)-3H-diazirin-3-yl]propanamido]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida
1.55
-
 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[3-(3-methyl-3H-diazirin-3-yl)propanamido]-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida
2.25
-
 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[2-[4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenyl]acetamido]-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida
2.95
-
 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-([5-[(3aR,6R,6aS)-2-oxohexahydro-1H-thieno[2,3-d]imidazol-6-yl]pentanoyl]amino)-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida
3.46
-
 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[(4-oxopentanoyl)amino]-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida
12.6
-
 2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida
18
-
 2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida
154
-
 2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida
216
-
 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose at pH 7.9 and 25°C Pseudomonas putida