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Literature summary for 2.7.1.221 extracted from

  • DeMeester, K.E.; Liang, H.; Jensen, M.R.; Jones, Z.S.; D'Ambrosio, E.A.; Scinto, S.L.; Zhou, J.; Grimes, C.L.
    Synthesis of functionalized N-acetyl muramic acids to probe bacterial cell wall recycling and biosynthesis (2018), J. Am. Chem. Soc., 140, 9458-9465 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.221

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain QKU Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0303
-
 2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida
0.55
-
 2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida
1.12
-
 2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-D-muramate Pseudomonas putida
-
 ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
 Pseudomonas putida ADP + 2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose
-
 ?
ATP + 2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
 Pseudomonas putida ADP + 2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose
-
 ?
ATP + 2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
 Pseudomonas putida ADP + 2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose
-
 ?
ATP + N-acetyl-D-muramate
-
 Pseudomonas putida ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
amgK
-
 Pseudomonas putida
MurNAc/GlcNAc anomeric kinase
-
 Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.67
-
 2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida
9.74
-
 2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida
14.07
-
 2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12.6
-
 2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida
18
-
 2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida
154
-
 2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose pH and temperature not specified in the publication Pseudomonas putida