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Literature summary for 2.7.1.22 extracted from

  • Grose, J.H.; Bergthorsson, U.; Roth, J.R.
    Regulation of NAD synthesis by the trifunctional NadR protein of Salmonella enterica (2005), J. Bacteriol., 187, 2774-2782.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.22

Cloned(Commentary)

Cloned (Comment) Organism
gene nadR, expression of N-terminally His6-tagged enzyme in Escherichia coli Salmonella enterica

Protein Variants

Protein Variants Comment Organism
additional information analysis of mutant strains for activities/functions of enzyme domains, reduced activity of class I mutants, overview Salmonella enterica

Inhibitors

Inhibitors Comment Organism Structure
NAD+ 69% inhibition at 1.2 mM, complete inhibition at 5 mM Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 N-ribosylnicotinamide recombinant enzyme, pH 7.6, 37°C Salmonella enterica
1.2
-
 ATP recombinant enzyme, pH 7.6, 37°C Salmonella enterica

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-ribosylnicotinamide Salmonella enterica activity is essential for N-ribosylnicotinamide assimilation ADP + nicotinamide ribonucleotide
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
 diverse strains with different genotypes, gene nadR
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography Salmonella enterica

Reaction

Reaction Comment Organism Reaction ID
ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide bifunctional enzyme exhibiting nicotinamide ribose kinase activity, EC 2.7.1.22, and weak adenylyltransferase activity, EC 2.7.7.1 Salmonella enterica
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4
-
 purified recombinant enzyme Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-ribosylnicotinamide
-
 Salmonella enterica ADP + nicotinamide ribonucleotide
-
 ?
ATP + N-ribosylnicotinamide activity is essential for N-ribosylnicotinamide assimilation Salmonella enterica ADP + nicotinamide ribonucleotide
-
 ?
additional information the enzymes' C-terminal domain has nicotinamide ribose kinase activity, the central domain performes adenylyltransferase reaction, EC 2.7.7.1, overview, the purified enzyme binds nadB promotor sequence in absence of ATP with or without NAD+, DNA binding activity of the enzyme is regulated by ATP binding to the adenylyltransferase domain Salmonella enterica ?
-
 ?

Synonyms

Synonyms Comment Organism
NadR
-
 Salmonella enterica
nicotinamide ribose kinase
-
 Salmonella enterica
NmR-K
-
 Salmonella enterica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Salmonella enterica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Salmonella enterica

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Salmonella enterica