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Literature summary for 2.7.1.22 extracted from

  • Kurnasov, O.V.; Polanuyer, B.M.; Ananta, S.; Sloutsky, R.; Tam, A.; Gerdes, S.Y.; Osterman, A.L.
    Ribosylnicotinamide kinase domain of NadR protein: identification and implications in NAD biosynthesis (2002), J. Bacteriol., 184, 6906-6917.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.22

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Salmonella enterica
expression in Escherichia coli Haemophilus influenzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 nicotinamide mononucleotide pH 7.5, 37°C, nicotinamide mononucleotide adenylyltransferase activity Haemophilus influenzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-ribosylnicotinamide Escherichia coli involved in NAD salvage pathway ADP + nicotinamide ribonucleotide
-
 ?
ATP + N-ribosylnicotinamide Salmonella enterica involved in NAD salvage pathway ADP + nicotinamide ribonucleotide
-
 ?
ATP + N-ribosylnicotinamide Haemophilus influenzae involved in NAD salvage pathway ADP + nicotinamide ribonucleotide
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Haemophilus influenzae Q57425
-
-
Salmonella enterica
-
 serovar typhimurium LT2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, Ni2+-nitrilotriacetic acid-agarose, Superdex 200 Escherichia coli
recombinant enzyme, Ni2+-nitrilotriacetic acid-agarose, Superdex 200 Salmonella enterica
recombinant enzyme, Ni2+-nitrilotriacetic acid-agarose, Superdex 200 Haemophilus influenzae

Reaction

Reaction Comment Organism Reaction ID
ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide enzyme has both ribosylnicotinamide kinase, i.e. EC 2.7.1.22, and nicotinamide mononucleotide adenylyltransferase, i.e. EC 2.7.7.18, activity Escherichia coli
a
ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide enzyme has both ribosylnicotinamide kinase, i.e. EC 2.7.1.22, and nicotinamide mononucleotide adenylyltransferase, i.e. EC 2.7.7.18, activity Salmonella enterica
a
ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide enzyme has both ribosylnicotinamide kinase, i.e. EC 2.7.1.22, and nicotinamide mononucleotide adenylyltransferase, i.e. EC 2.7.7.18, activity Haemophilus influenzae
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
-
 Salmonella enterica
0.04
-
 pH 7.5, 37°C, nicotinamide mononucleotide adenylyltransferase activity Haemophilus influenzae
0.04
-
 pH 7.5, 37°C, ribosylnicotinamide kinase activity Haemophilus influenzae
0.9
-
 pH 7.5, 37°C, nicotinamide mononucleotide adenylyltransferase activity Haemophilus influenzae
1
-
 pH 7.5, 37°C, ribosylnicotinamide kinase activity Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-ribosylnicotinamide
-
 Escherichia coli ADP + nicotinamide ribonucleotide
-
 ?
ATP + N-ribosylnicotinamide
-
 Salmonella enterica ADP + nicotinamide ribonucleotide
-
 ?
ATP + N-ribosylnicotinamide no activity with N-ribosylnicotinate Haemophilus influenzae ADP + nicotinamide ribonucleotide
-
 ?
ATP + N-ribosylnicotinamide involved in NAD salvage pathway Escherichia coli ADP + nicotinamide ribonucleotide
-
 ?
ATP + N-ribosylnicotinamide involved in NAD salvage pathway Salmonella enterica ADP + nicotinamide ribonucleotide
-
 ?
ATP + N-ribosylnicotinamide involved in NAD salvage pathway Haemophilus influenzae ADP + nicotinamide ribonucleotide
-
 ?
ATP + nicotinamide mononucleotide
-
 Escherichia coli NAD+ + diphosphate
-
 r
ATP + nicotinamide mononucleotide
-
 Salmonella enterica NAD+ + diphosphate
-
 r
ATP + nicotinamide mononucleotide
-
 Haemophilus influenzae NAD+ + diphosphate
-
 r

Synonyms

Synonyms Comment Organism
NadR
-
 Escherichia coli
NadR
-
 Salmonella enterica
NadR
-
 Haemophilus influenzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.92
-
 nicotinamide mononucleotide pH 7.5, 37°C, nicotinamide mononucleotide adenylyltransferase activity Haemophilus influenzae