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Literature summary for 2.7.1.213 extracted from
- Nucleoside kinases in adult Schistosoma mansoni: phosphorylation of pyrimidine nucleosides (2014), Mol. Biochem. Parasitol., 194, 53-55.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| uridine | competitive inhibition | Schistosoma mansoni |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.073 | - |
cytidine | pH 7.4, 20-40°C, with ATP | Schistosoma mansoni | |
| 2.1 | - |
ATP | pH 7.4, 20-40°C | Schistosoma mansoni | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Schistosoma mansoni | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Schistosoma mansoni | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schistosoma mansoni | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially, separation of cytosolic cytidine and uridine kinases by anion exchange chromatography | Schistosoma mansoni |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| Storage Stability | Organism |
|---|---|
| upon freezing at -20°C all activity is lost from the cytosol within a few hours, addition of PMSF preserved kinase activities for a week at 4°C and well over 3 weeks at -80°C | Schistosoma mansoni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cytidine + ATP | - |
Schistosoma mansoni | CMP + ADP | - |
? | |
| cytidine + CTP | - |
Schistosoma mansoni | CMP + CDP | - |
? | |
| cytidine + GTP | preferred cofactor | Schistosoma mansoni | CMP + GDP | - |
? | |
| cytidine + UTP | - |
Schistosoma mansoni | CMP + UDP | - |
? | |
| additional information | cytidine kinase is specific for cytidine, but various nucleoside 5'-triphosphate act as phosphate donors for the kinase albeit to different degrees, overview. Poor activity with dTTP, no activity with AMP and 4-nitrophenyl phosphate | Schistosoma mansoni | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | assay at | Schistosoma mansoni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Schistosoma mansoni |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Schistosoma mansoni | |
| CTP | - |
Schistosoma mansoni | |
| GTP | preferred cofactor | Schistosoma mansoni | |
| UTP | - |
Schistosoma mansoni | |
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Release 2023.1 (January 2023)
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