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Literature summary for 2.7.1.21 extracted from
- Exploiting temperature-dependent substrate promiscuity for nucleoside analogue activation by thymidine kinase from Thermotoga maritima (2007), J. Am. Chem. Soc., 129, 8714-8715 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| heterologously overexpressed in Escherichia coli | Thermotoga maritima |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
thymidine | 37°C, pH not specified in the publication | Thermotoga maritima |  |
| 0.00065 | - |
2',3'-dideoxy-3'-azidothymidine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 0.001 | - |
5-fluoro-2'-deoxyuridine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 0.001 | - |
thymidine | 82°C, pH not specified in the publication | Thermotoga maritima | |
| 0.0015 | - |
2'-deoxyuridine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 0.0087 | - |
2',3'-dideoxy-3'-azidothymidine | 82°C, pH not specified in the publication | Thermotoga maritima | |
| 0.03 | - |
2',3'-dideoxythymidine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 0.04 | - |
ATP | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 0.075 | - |
2',3'-dideoxy-2',3'-didehydrothymidine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 0.114 | - |
dioxolane thymidine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 0.132 | - |
2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 0.187 | - |
ATP | 82°C, pH not specified in the publication | Thermotoga maritima | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | Q9WYN2 | - |
- |
| Thermotoga maritima DSM 3109 | Q9WYN2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2',3'-dideoxy-2',3'-didehydrothymidine | - |
Thermotoga maritima | ADP + 2',3'-dideoxy-2',3'-didehydrothymidine 5'-phosphate | - |
? | |
| ATP + 2',3'-dideoxy-3'-azidothymidine | - |
Thermotoga maritima | ADP + 2',3'-dideoxy-3'-azidothymidine 5'-phosphate | - |
? | |
| ATP + 2',3'-dideoxythymidine | - |
Thermotoga maritima | ADP + 2',3'-dideoxythymidine 5'-phosphate | - |
? | |
| ATP + 2',3'-dideoxythymidine | - |
Thermotoga maritima DSM 3109 | ADP + 2',3'-dideoxythymidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyuridine | - |
Thermotoga maritima | ADP + 2'-deoxyuridine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyuridine | - |
Thermotoga maritima DSM 3109 | ADP + 2'-deoxyuridine 5'-phosphate | - |
? | |
| ATP + 2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil | - |
Thermotoga maritima | ADP + 2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil 5'-phosphate | - |
? | |
| ATP + 5-fluoro-2'-deoxyuridine | - |
Thermotoga maritima | ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate | - |
? | |
| ATP + 5-fluoro-2'-deoxyuridine | - |
Thermotoga maritima DSM 3109 | ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate | - |
? | |
| ATP + dioxolane thymidine | - |
Thermotoga maritima | ADP + dioxolane thymidine 5'-phosphate | - |
? | |
| ATP + thymidine | - |
Thermotoga maritima | ADP + dTMP | - |
? | |
| ATP + thymidine | - |
Thermotoga maritima DSM 3109 | ADP + dTMP | - |
? | |
| additional information | no activity with uridine, L-thymidine or 2',3'-dideoxyuridine | Thermotoga maritima | ? | - |
? | |
| additional information | no activity with uridine, L-thymidine or 2',3'-dideoxyuridine | Thermotoga maritima DSM 3109 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | - |
Thermotoga maritima |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.29 | - |
2',3'-dideoxy-3'-azidothymidine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 0.29 | - |
dioxolane thymidine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 0.3 | - |
thymidine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 0.33 | - |
2',3'-dideoxythymidine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 0.4 | - |
ATP | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 0.41 | - |
2',3'-dideoxy-2',3'-didehydrothymidine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 0.5 | - |
2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 0.7 | - |
2'-deoxyuridine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 0.74 | - |
5-fluoro-2'-deoxyuridine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 1.6 | - |
2',3'-dideoxy-3'-azidothymidine | 82°C, pH not specified in the publication | Thermotoga maritima | |
| 3.5 | - |
ATP | 82°C, pH not specified in the publication | Thermotoga maritima | |
| 3.5 | - |
thymidine | 82°C, pH not specified in the publication | Thermotoga maritima | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
dioxolane thymidine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 3.8 | - |
2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 5.4 | - |
2',3'-dideoxy-2',3'-didehydrothymidine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 10 | - |
ATP | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 11 | - |
2',3'-dideoxythymidine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 18 | - |
ATP | 82°C, pH not specified in the publication | Thermotoga maritima | |
| 180 | - |
2',3'-dideoxy-3'-azidothymidine | 82°C, pH not specified in the publication | Thermotoga maritima | |
| 450 | - |
2',3'-dideoxy-3'-azidothymidine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 480 | - |
2'-deoxyuridine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 600 | - |
thymidine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 740 | - |
5-fluoro-2'-deoxyuridine | 37°C, pH not specified in the publication | Thermotoga maritima | |
| 3500 | - |
thymidine | 82°C, pH not specified in the publication | Thermotoga maritima | |
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