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Literature summary for 2.7.1.21 extracted from

  • Mutahir, Z.; Clausen, A.R.; Andersson, K.M.; Wisen, S.M.; Munch-Petersen, B.; Piskur, J.
    Thymidine kinase 1 regulatory fine-tuning through tetramer formation (2013), FEBS J., 280, 1531-1541.
    View publication on PubMed
Search for more literature for 2.7.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens
expressed in Escherichia coli BL21(DE3) cells Dictyostelium discoideum
expressed in Escherichia coli BL21(DE3) cells Gallus gallus
expressed in Escherichia coli BL21(DE3) cells Rattus norvegicus
expressed in Escherichia coli BL21(DE3) cells Bacillus cereus
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana
expressed in Escherichia coli BL21(DE3) cells Caenorhabditis elegans
expressed in Escherichia coli BL21(DE3) cells Danio rerio
expressed in Escherichia coli BL21(DE3) cells Xenopus tropicalis
expressed in Escherichia coli BL21(DE3) cells Anolis carolinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Rattus norvegicus
0.0005
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Homo sapiens
0.0006
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Danio rerio
0.0006
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Bacillus cereus
0.0006
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Caenorhabditis elegans
0.0006
-
 thymidine subunit alpha, in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Arabidopsis thaliana
0.0007
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Bacillus cereus
0.0007
-
 thymidine subunit alpha, in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Arabidopsis thaliana
0.0009
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Caenorhabditis elegans
0.0009
-
 thymidine subunit beta, in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Arabidopsis thaliana
0.0009
-
 thymidine subunit beta, in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Arabidopsis thaliana
0.0012
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Gallus gallus
0.0014
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Danio rerio
0.0016
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Xenopus tropicalis
0.002
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Anolis carolinensis
0.0024
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Dictyostelium discoideum
0.0025
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Anolis carolinensis
0.008
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Dictyostelium discoideum
0.012
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Gallus gallus
0.016
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Homo sapiens
0.017
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Rattus norvegicus
0.017
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Xenopus tropicalis

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21700
-
 2 * 21700, calculated from amino acid sequence Bacillus cereus
24800
-
 4 * 24800, calculated from amino acid sequence Gallus gallus
25400
-
 2 * 25400, calculated from amino acid sequence Dictyostelium discoideum
25400
-
 4 * 25400, calculated from amino acid sequence Homo sapiens
25600
-
 4 * 25600, calculated from amino acid sequence Danio rerio
25600
-
 4 * 25600, calculated from amino acid sequence Anolis carolinensis
25700
-
 4 * 25700, calculated from amino acid sequence Rattus norvegicus
26000
-
 2 * 26000 + 2 * 30700, calculated from amino acid sequence Arabidopsis thaliana
26300
-
 4 * 26300, calculated from amino acid sequence Xenopus tropicalis
29700
-
 4 * 29700, calculated from amino acid sequence Caenorhabditis elegans
30700
-
 2 * 26000 + 2 * 30700, calculated from amino acid sequence Arabidopsis thaliana
32000
-
 for subunit alpha pre-incubated without ATP, two different peaks at 52000 and 32000 Da are obtained during gel filtration Arabidopsis thaliana
43000
-
 gel filtration Bacillus cereus
46000
-
 gel filtration Gallus gallus
49000
-
 subunit beta, gel filtration Arabidopsis thaliana
52000
-
 for subunit alpha pre-incubated without ATP, two different peaks at 52000 and 32000 Da are obtained during gel filtration Arabidopsis thaliana
55000
-
 gel filtration Rattus norvegicus
57000
-
 gel filtration Dictyostelium discoideum
57000
-
 gel filtration Anolis carolinensis
58000
-
 gel filtration Homo sapiens
64000
-
 for enzyme pre-incubated without ATP, two different peaks at 98000 and 64000 Da are obtained during gel filtration Xenopus tropicalis
82000
-
 gel filtration Danio rerio
98000
-
 for enzyme pre-incubated without ATP, two different peaks at 98000 and 64000 Da are obtained during gel filtration Xenopus tropicalis
130000
-
 gel filtration Caenorhabditis elegans

Organism

Organism UniProt Comment Textmining
Anolis carolinensis C3VAL3
-
-
Arabidopsis thaliana Q9S750 and Q9FN47 subunits alpha and beta
-
Bacillus cereus Q0H0H6
-
-
Caenorhabditis elegans G5EEZ5
-
-
Danio rerio Q803Y1
-
-
Dictyostelium discoideum Q27564
-
-
Gallus gallus P04047
-
-
Homo sapiens P04183
-
-
Rattus norvegicus P27158
-
-
Xenopus tropicalis Q5I0A2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + thymidine
-
 Homo sapiens ADP + dTMP
-
 ?
ATP + thymidine
-
 Dictyostelium discoideum ADP + dTMP
-
 ?
ATP + thymidine
-
 Gallus gallus ADP + dTMP
-
 ?
ATP + thymidine
-
 Rattus norvegicus ADP + dTMP
-
 ?
ATP + thymidine
-
 Bacillus cereus ADP + dTMP
-
 ?
ATP + thymidine
-
 Arabidopsis thaliana ADP + dTMP
-
 ?
ATP + thymidine
-
 Caenorhabditis elegans ADP + dTMP
-
 ?
ATP + thymidine
-
 Danio rerio ADP + dTMP
-
 ?
ATP + thymidine
-
 Xenopus tropicalis ADP + dTMP
-
 ?
ATP + thymidine
-
 Anolis carolinensis ADP + dTMP
-
 ?

Subunits

Subunits Comment Organism
heterotetramer 2 * 26000 + 2 * 30700, calculated from amino acid sequence Arabidopsis thaliana
homodimer 2 * 21700, calculated from amino acid sequence Bacillus cereus
homodimer 2 * 25400, calculated from amino acid sequence Dictyostelium discoideum
homotetramer 4 * 24800, calculated from amino acid sequence Gallus gallus
homotetramer 4 * 25400, calculated from amino acid sequence Homo sapiens
homotetramer 4 * 25600, calculated from amino acid sequence Danio rerio
homotetramer 4 * 25600, calculated from amino acid sequence Anolis carolinensis
homotetramer 4 * 25700, calculated from amino acid sequence Rattus norvegicus
homotetramer 4 * 26300, calculated from amino acid sequence Xenopus tropicalis
homotetramer 4 * 29700, calculated from amino acid sequence Caenorhabditis elegans

Synonyms

Synonyms Comment Organism
thymidine kinase 1
-
 Homo sapiens
thymidine kinase 1
-
 Dictyostelium discoideum
thymidine kinase 1
-
 Gallus gallus
thymidine kinase 1
-
 Rattus norvegicus
thymidine kinase 1
-
 Bacillus cereus
thymidine kinase 1
-
 Arabidopsis thaliana
thymidine kinase 1
-
 Caenorhabditis elegans
thymidine kinase 1
-
 Danio rerio
thymidine kinase 1
-
 Xenopus tropicalis
thymidine kinase 1
-
 Anolis carolinensis
TK1
-
 Homo sapiens
TK1
-
 Dictyostelium discoideum
TK1
-
 Gallus gallus
TK1
-
 Rattus norvegicus
TK1
-
 Bacillus cereus
TK1
-
 Arabidopsis thaliana
TK1
-
 Caenorhabditis elegans
TK1
-
 Danio rerio
TK1
-
 Xenopus tropicalis
TK1
-
 Anolis carolinensis