Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3) cells as a fusion protein to glutathione transferase | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H13I | the mutant shows strongly increased Km compared to the wild type enzyme | Human alphaherpesvirus 1 |
H13I/Y127F | the mutant shows increased Km compared to the wild type enzyme | Human alphaherpesvirus 1 |
I59H | the mutant shows imcreased Km compared to the wild type enzyme | Homo sapiens |
I59L | the mutant shows imcreased Km compared to the wild type enzyme | Homo sapiens |
I59V | the mutant shows imcreased Km compared to the wild type enzyme | Homo sapiens |
I59Y | the mutant shows decreased Km compared to the wild type enzyme | Homo sapiens |
P12N | the mutant shows increased Km compared to the wild type enzyme | Human alphaherpesvirus 1 |
P12N/H13I | the mutant shows decreased Km compared to the wild type enzyme | Human alphaherpesvirus 1 |
P12N/H13I/Y127F | the mutant shows strongly increased Km compared to the wild type enzyme | Human alphaherpesvirus 1 |
P12N/Y127F | the mutant shows strongly increased Km compared to the wild type enzyme | Human alphaherpesvirus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(3,4-dichlorobenzyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | - |
Homo sapiens | |
1-(3,4-dichlorobenzyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | - |
Human alphaherpesvirus 1 | |
1-(3,4-dichlorophenyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | - |
Homo sapiens | |
1-(3,4-dichlorophenyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | - |
Human alphaherpesvirus 1 | |
1-benzyl-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | - |
Homo sapiens | |
1-benzyl-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | - |
Human alphaherpesvirus 1 | |
1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]thiourea | - |
Homo sapiens | |
1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]thiourea | - |
Human alphaherpesvirus 1 | |
1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]urea | - |
Homo sapiens | |
1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]urea | - |
Human alphaherpesvirus 1 | |
1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | - |
Homo sapiens | |
1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | - |
Human alphaherpesvirus 1 | |
1-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]-3-phenylthiourea | - |
Homo sapiens | |
1-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]-3-phenylthiourea | - |
Human alphaherpesvirus 1 | |
3'-([[(4-chlorophenyl)carbamothioyl]amino]methyl)-3'-deoxythymidine | - |
Homo sapiens | |
3'-([[(4-chlorophenyl)carbamothioyl]amino]methyl)-3'-deoxythymidine | - |
Human alphaherpesvirus 1 | |
3'-deoxy-3'-([[(3,4-dichlorophenyl)carbamothioyl]amino]methyl)thymidine | - |
Homo sapiens | |
3'-deoxy-3'-([[(3,4-dichlorophenyl)carbamothioyl]amino]methyl)thymidine | - |
Human alphaherpesvirus 1 | |
3'-deoxy-3'-([[(4-methoxyphenyl)carbamothioyl]amino]methyl)thymidine | - |
Homo sapiens | |
3'-deoxy-3'-([[(4-methoxyphenyl)carbamothioyl]amino]methyl)thymidine | - |
Human alphaherpesvirus 1 | |
3'-deoxy-3'-([[(4-methylphenyl)carbamothioyl]amino]methyl)thymidine | - |
Homo sapiens | |
3'-deoxy-3'-([[(4-methylphenyl)carbamothioyl]amino]methyl)thymidine | - |
Human alphaherpesvirus 1 | |
3'-[([[4-(benzyloxy)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine | - |
Homo sapiens | |
3'-[([[4-(benzyloxy)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine | - |
Human alphaherpesvirus 1 | |
3'-[([[4-chloro-3-(trifluoromethyl)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine | - |
Homo sapiens | |
3'-[([[4-chloro-3-(trifluoromethyl)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine | - |
Human alphaherpesvirus 1 | |
5'-deoxy-5'-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamothioyl)amino]thymidine | - |
Homo sapiens | |
5'-deoxy-5'-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamothioyl)amino]thymidine | - |
Human alphaherpesvirus 1 | |
5'-deoxy-5'-[[(diphenylmethyl)carbamothioyl]amino]thymidine | - |
Homo sapiens | |
5'-deoxy-5'-[[(diphenylmethyl)carbamothioyl]amino]thymidine | - |
Human alphaherpesvirus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00023 | - |
thymidine | mutant enzyme P12N/H13I, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Human alphaherpesvirus 1 | |
0.00044 | - |
thymidine | wild type enzyme, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Human alphaherpesvirus 1 | |
0.0039 | - |
thymidine | mutant enzyme P12N, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Human alphaherpesvirus 1 | |
0.0039 | - |
thymidine | thymidine kinase-2 mutant enzyme I59Y, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.0043 | - |
thymidine | wild type thymidine kinase-2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.0051 | - |
thymidine | thymidine kinase-2 mutant enzyme I59V, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.0056 | - |
thymidine | thymidine kinase-2 mutant enzyme I59L, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.006 | - |
thymidine | thymidine kinase-2 mutant enzyme I59H, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.037 | - |
thymidine | mutant enzyme H13I/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Human alphaherpesvirus 1 | |
0.263 | - |
thymidine | mutant enzyme P12N/H13I/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Human alphaherpesvirus 1 | |
0.59 | - |
thymidine | mutant enzyme P12N/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Human alphaherpesvirus 1 | |
0.612 | - |
thymidine | mutant enzyme H13I, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Human alphaherpesvirus 1 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | beta-thymidine kinase-2 | Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Human alphaherpesvirus 1 | P03176 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + thymidine | - |
Homo sapiens | ADP + thymidine 5'-phosphate | - |
? | |
ATP + thymidine | - |
Human alphaherpesvirus 1 | ADP + thymidine 5'-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-thymidine kinase-1 | - |
Homo sapiens |
beta-thymidine kinase-2 | - |
Homo sapiens |
dThd kinase-1 | - |
Homo sapiens |
dThd kinase-2 | - |
Homo sapiens |
thymidine kinase-1 | - |
Homo sapiens |
thymidine kinase-2 | - |
Homo sapiens |
TK-1 | - |
Homo sapiens |
TK-2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
ATP | - |
Human alphaherpesvirus 1 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00015 | - |
- |
Homo sapiens | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]thiourea | |
0.00042 | - |
- |
Homo sapiens | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]urea | |
0.00043 | - |
- |
Homo sapiens | 3'-[([[4-(benzyloxy)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine | |
0.00047 | - |
- |
Homo sapiens | 3'-[([[4-chloro-3-(trifluoromethyl)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine | |
0.00064 | - |
- |
Homo sapiens | 3'-deoxy-3'-([[(3,4-dichlorophenyl)carbamothioyl]amino]methyl)thymidine | |
0.0012 | - |
- |
Homo sapiens | 3'-([[(4-chlorophenyl)carbamothioyl]amino]methyl)-3'-deoxythymidine | |
0.0012 | - |
- |
Homo sapiens | 3'-deoxy-3'-([[(4-methylphenyl)carbamothioyl]amino]methyl)thymidine | |
0.0031 | - |
- |
Homo sapiens | 3'-deoxy-3'-([[(4-methoxyphenyl)carbamothioyl]amino]methyl)thymidine | |
0.031 | - |
- |
Homo sapiens | 5'-deoxy-5'-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamothioyl)amino]thymidine | |
0.038 | - |
- |
Homo sapiens | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | |
0.041 | - |
- |
Homo sapiens | 1-(3,4-dichlorobenzyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | |
0.046 | - |
- |
Human alphaherpesvirus 1 | 3'-[([[4-chloro-3-(trifluoromethyl)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine | |
0.057 | - |
- |
Homo sapiens | 1-(3,4-dichlorophenyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | |
0.087 | - |
- |
Human alphaherpesvirus 1 | 3'-deoxy-3'-([[(3,4-dichlorophenyl)carbamothioyl]amino]methyl)thymidine | |
0.099 | - |
- |
Homo sapiens | 1-benzyl-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | |
0.11 | - |
- |
Homo sapiens | 5'-deoxy-5'-[[(diphenylmethyl)carbamothioyl]amino]thymidine | |
0.195 | - |
- |
Human alphaherpesvirus 1 | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]thiourea | |
0.232 | - |
- |
Human alphaherpesvirus 1 | 3'-[([[4-(benzyloxy)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine | |
0.335 | - |
- |
Human alphaherpesvirus 1 | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]urea | |
0.365 | - |
- |
Human alphaherpesvirus 1 | 1-(3,4-dichlorobenzyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | |
0.392 | - |
- |
Human alphaherpesvirus 1 | 5'-deoxy-5'-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamothioyl)amino]thymidine | |
0.4 | - |
- |
Human alphaherpesvirus 1 | 3'-([[(4-chlorophenyl)carbamothioyl]amino]methyl)-3'-deoxythymidine | |
0.405 | - |
- |
Homo sapiens | 1-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]-3-phenylthiourea | |
0.422 | - |
- |
Human alphaherpesvirus 1 | 1-(3,4-dichlorophenyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | |
0.444 | - |
- |
Human alphaherpesvirus 1 | 3'-deoxy-3'-([[(4-methylphenyl)carbamothioyl]amino]methyl)thymidine | |
0.472 | - |
- |
Human alphaherpesvirus 1 | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | |
0.5 | - |
IC50 above 0.5 mM | Human alphaherpesvirus 1 | 3'-deoxy-3'-([[(4-methoxyphenyl)carbamothioyl]amino]methyl)thymidine | |
0.5 | - |
IC50 above 0.5 mM | Human alphaherpesvirus 1 | 1-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]-3-phenylthiourea | |
0.5 | - |
IC50 above 0.5 mM | Human alphaherpesvirus 1 | 5'-deoxy-5'-[[(diphenylmethyl)carbamothioyl]amino]thymidine | |
0.5 | - |
IC50 above 0.5 mM | Human alphaherpesvirus 1 | 1-benzyl-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea |