Application | Comment | Organism |
---|---|---|
medicine | enzyme can be useful in gene therapy | Ureaplasma urealyticum |
medicine | enzyme can be useful in gene therapy | Homo sapiens |
synthesis | enzyme can be useful in drug design | Ureaplasma urealyticum |
synthesis | enzyme can be useful in drug design | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of C-terminally truncated enzyme lacking 41 amino acid residues | Homo sapiens |
gene tdk, expression in Escherichia coli as wild-type and selenomethionine-labeled enzymes | Ureaplasma urealyticum |
Crystallization (Comment) | Organism |
---|---|
purified recombinant C-terminally truncated enzyme in complex with inhibitor dTTP, hanging drop vapour diffusion method, 7-13 mg/ml protein in solution with 5 mM dTTP mixed with an equal volume of crystallization solution containing 0.1 M Na-citrate, pH 5.6, 20% 2-propanol, and 14-16% PEG 4000, 15°C, X-ray diffraction structure determination and analysis at 2.4-3.5 A resolution | Homo sapiens |
purified recombinant wild-type and selenomethionine-labeled enzyme in complex with inhibitor dTTP, hanging drop vapour diffusion method, 8 mg/ml protein in solution with 5 mM dTTp mixed with an equal volume of reservoir solution containing 19% PEG 2000, 0.2 M LiCl, 5 mM DTT, and 0.1 M MES, pH 6.0, 15°C, X-ray diffraction structure determination and analysis at 2.5 or 3.0 A resolution, respectively | Ureaplasma urealyticum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTTP | competitive feedback inhibition, binding structure analysis | Homo sapiens | |
dTTP | competitive feedback inhibition, binding structure analysis | Ureaplasma urealyticum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Ureaplasma urealyticum | 5829 | - |
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Ureaplasma urealyticum | |
Mg2+ | - |
Homo sapiens | |
Zn2+ | at a molar ratio of 0.51, connects beta-structures at the root of a ribbon that forms a stem which widens to a lasso-loop | Ureaplasma urealyticum | |
Zn2+ | at a molar ratio of 0.56, connects beta-structures at the root of a ribbon that forms a stem which widens to a lasso-loop | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxythymidine | Ureaplasma urealyticum | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
ir | |
ATP + 2'-deoxythymidine | Homo sapiens | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
ir | |
ATP + 2'-deoxyuridine | Ureaplasma urealyticum | - |
ADP + 2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + 2'-deoxyuridine | Homo sapiens | - |
ADP + 2'-deoxyuridine 5'-phosphate | - |
? | |
additional information | Homo sapiens | the cell-cycle-regulated enzyme is important in nucleotide metabolism and an activator of antiviral and anticancer drugs | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P04183 | - |
- |
Ureaplasma urealyticum | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + thymidine = ADP + dTMP | substrate binding and active site structure | Ureaplasma urealyticum | |
ATP + thymidine = ADP + dTMP | substrate binding and active site structure | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxythymidine | - |
Ureaplasma urealyticum | ADP + 2'-deoxythymidine 5'-phosphate | - |
ir | |
ATP + 2'-deoxythymidine | - |
Homo sapiens | ADP + 2'-deoxythymidine 5'-phosphate | - |
ir | |
ATP + 2'-deoxyuridine | - |
Ureaplasma urealyticum | ADP + 2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + 2'-deoxyuridine | - |
Homo sapiens | ADP + 2'-deoxyuridine 5'-phosphate | - |
? | |
additional information | the cell-cycle-regulated enzyme is important in nucleotide metabolism and an activator of antiviral and anticancer drugs | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | each subunit contains an alpha/beta-domain, and a domain with a structural zinc ion | Ureaplasma urealyticum |
tetramer | each subunit contains an alpha/beta-domain, and a domain with a structural zinc ion | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the thymidine kinase 1, i.e. TK1, family of enzymes | Ureaplasma urealyticum |
More | the enzyme belongs to the thymidine kinase 1, i.e. TK1, family of enzymes | Homo sapiens |
thymidine kinase 1 | - |
Ureaplasma urealyticum |
thymidine kinase 1 | - |
Homo sapiens |
TK1 | - |
Ureaplasma urealyticum |
TK1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Ureaplasma urealyticum | |
ATP | - |
Homo sapiens |