Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Human alphaherpesvirus 1 |
Protein Variants | Comment | Organism |
---|---|---|
A175V | site-sirected mutagenesis, inactive mutant, mutation of residue 51 might in vivo be involved into generation of acyclovir resistance of HSV | Human alphaherpesvirus 1 |
D77N | site-sirected mutagenesis, mutant activity is slightly increased compared to the wild-type enzyme | Human alphaherpesvirus 1 |
E83K | site-sirected mutagenesis, inactive mutant, mutation of residue 51 might in vivo be involved into generation of acyclovir resistance of HSV | Human alphaherpesvirus 1 |
R51W | site-sirected mutagenesis, inactive mutant, mutation of residue 51 might in vivo be involved into generation of acyclovir resistance of HSV | Human alphaherpesvirus 1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Human alphaherpesvirus 1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acyclovir | Human alphaherpesvirus 1 | drug activation by phosphorylation, some naturally occurring mutations lead to inability of acyclovir phosphorylation and thus resistance against the drug | ADP + acyclovir phosphate | - |
? | |
ATP + thymidine | Human alphaherpesvirus 1 | - |
ADP + thymidine 5'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human alphaherpesvirus 1 | - |
i.e. HSV-1, acyclovir-sensitive strain KOS, genetic polymorphism | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Human alphaherpesvirus 1 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + thymidine = ADP + dTMP | the amino acid residues at positions 51, 83, and 185 are important for enzyme activity | Human alphaherpesvirus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acyclovir | drug activation by phosphorylation | Human alphaherpesvirus 1 | ADP + acyclovir phosphate | - |
? | |
ATP + acyclovir | drug activation by phosphorylation, some naturally occurring mutations lead to inability of acyclovir phosphorylation and thus resistance against the drug | Human alphaherpesvirus 1 | ADP + acyclovir phosphate | - |
? | |
ATP + thymidine | - |
Human alphaherpesvirus 1 | ADP + thymidine 5'-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TK | - |
Human alphaherpesvirus 1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Human alphaherpesvirus 1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Human alphaherpesvirus 1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Human alphaherpesvirus 1 |