Cloned (Comment) | Organism |
---|---|
TK2 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H121N | similar subunit structure to wild-type enzyme, decreased enzyme activity | Homo sapiens |
I212N | similar subunit structure to wild-type enzyme, less than 1% activity compared to wild-type | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dCTP | - |
Homo sapiens | |
deoxcytidine | - |
Homo sapiens | |
dTTP | - |
Homo sapiens | |
thymidine | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0057 | - |
thymidine | H121N mutant, pH 7.6 | Homo sapiens | |
0.011 | - |
deoxycytidine | wild-type and H121N mutant, pH 7.6 | Homo sapiens | |
0.013 | - |
thymidine | wild-type, pH 7.6 | Homo sapiens | |
0.018 | - |
thymidine | I212 mutant, pH 7.6 | Homo sapiens | |
0.025 | - |
ATP | wild-type, thymidine as substrate, pH 7.6 | Homo sapiens | |
0.037 | - |
ATP | wild-type, deoxycytidine as substrate, pH 7.6 | Homo sapiens | |
0.059 | - |
ATP | I212 mutant, deoxycytidine as substrate, pH 7.6 | Homo sapiens | |
0.08 | - |
ATP | H121N mutant, thymidine as substrate, pH 7.6 | Homo sapiens | |
0.095 | - |
ATP | I212 mutant, thymidine as substrate, pH 7.6 | Homo sapiens | |
0.162 | - |
ATP | H121N mutant, deoxycytidine as substrate, pH 7.6 | Homo sapiens | |
0.568 | - |
deoxycytidine | I212 mutant, pH 7.6 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00142 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxycytidine | - |
Homo sapiens | ADP + 2'-deoxycytidine 5'-phosphate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00036 | - |
dCTP | H121N mutant, pH 7.6, competitive with thymidine as substrate | Homo sapiens | |
0.00079 | - |
dCTP | wild-type enzyme, pH 7.6, competitive with thymidine as substrate | Homo sapiens | |
0.00087 | - |
dCTP | wild-type enzyme, pH 7.6, competitive with deoxycytidine as substrate | Homo sapiens | |
0.00089 | - |
dTTP | H121N mutant, pH 7.6, competitive with thymidine as substrate | Homo sapiens | |
0.00089 | - |
dCTP | H121N mutant, pH 7.6, competitive with deoxycytidine as substrate | Homo sapiens | |
0.002 | - |
dTTP | wild-type enzyme, pH 7.6, competitive with thymidine as substrate | Homo sapiens | |
0.0025 | - |
dTTP | wild-type and H121N mutant, pH 7.6, non-competitive with deoxycytidine as substrate | Homo sapiens | |
0.0036 | - |
thymidine | H121N mutant, pH 7.6, competitive with deoxycytidine as substrate | Homo sapiens | |
0.004 | - |
deoxycytidine | H121N mutant, pH 7.6, non-competitive with thymidine as substrate | Homo sapiens | |
0.0049 | - |
thymidine | wild-type enzyme, pH 7.6, competitive with deoxycytidine as substrate | Homo sapiens | |
0.04 | - |
deoxycytidine | wild-type enzyme, pH 7.6, non-competitive with thymidine as substrate | Homo sapiens |