General Stability | Organism |
---|---|
stability decreases with increasing purity, ATP, thymidine, Mg2+ protect | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-deoxy-3'-aminothymidine | 5 mM | Mus musculus | |
2-mercaptoethanol | 5 mM | Mus musculus | |
5'-aminothymidine | - |
Mus musculus | |
dTTP | - |
Mus musculus | |
additional information | 5'-derivatives of thymidine | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
thymidine | pH 7.8, 37°C | Mus musculus | |
0.15 | - |
CTP | ATP | Mus musculus | |
0.15 | - |
ATP | pH 7.8, 37°C | Mus musculus | |
0.3 | - |
ATP | GTP | Mus musculus | |
0.3 | - |
GTP | pH 7.8, 37°C | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
sarcoma cell | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.053 | - |
- |
Mus musculus |
Storage Stability | Organism |
---|---|
-20°C, 0.1 mM thymidine, 1 week, 30% loss of activity | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + thymidine | ATP can be substituted by GTP | Mus musculus | ADP + dTMP | - |
? | |
ATP + thymidine | ATP can be substituted by dGTP | Mus musculus | ADP + dTMP | - |
? | |
ATP + thymidine | ATP can be substituted by ATP-arabinoside | Mus musculus | ADP + dTMP | - |
? | |
ATP + thymidine | ATP can be substituted by UTP | Mus musculus | ADP + dTMP | - |
? | |
ATP + thymidine | ATP can be substituted by dCTP | Mus musculus | ADP + dTMP | - |
? | |
CTP + thymidine | - |
Mus musculus | CDP + thymidine 5'-phosphate | - |
? | |
GTP + thymidine | - |
Mus musculus | GDP + thymidine 5'-phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
ATP, thymidine, Mg2+ protect against thermal inactivation | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Mus musculus | |
0.003 | - |
2'-deoxy-3'-aminothymidine | pH 7.8, 37°C | Mus musculus | |
3 | - |
5'-aminothymidine | pH 7.8, 37°C | Mus musculus |