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Literature summary for 2.7.1.21 extracted from
- Purification and characterization of herpes simplex virus (type 1) thymidine kinase produced in Escherichia coli by a high efficiency expression plasmid utilizing a lambda PL promoter and cI857 temperature-sensitive repressor (1983), J. Biol. Chem., 258, 11571-11575.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Herpes simplex virus |
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol protects against thermal inactivation | Herpes simplex virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acyclovir | syn. 9-[(2-hydroxyethoxy)methyl]guanine | Herpes simplex virus |  |
| iododeoxycytidine | - |
Herpes simplex virus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
thymidine | - |
Herpes simplex virus | |
| 0.118 | - |
ATP | - |
Herpes simplex virus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
2 * 42000, SDS-PAGE | Herpes simplex virus |
| 70000 | 80000 | gel filtration | Herpes simplex virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Herpes simplex virus | - |
type 1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Herpes simplex virus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.13 | - |
- |
Herpes simplex virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + thymidine | ATP can be substituted by GTP | Herpes simplex virus | ADP + dTMP | - |
? | |
| ATP + thymidine | ATP can be substituted by dGTP | Herpes simplex virus | ADP + dTMP | - |
? | |
| ATP + thymidine | ATP can be substituted by dCTP | Herpes simplex virus | ADP + dTMP | - |
? | |
| ATP + thymidine | ATP can be substituted by dATP | Herpes simplex virus | ADP + dTMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42000, SDS-PAGE | Herpes simplex virus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
2-mercaptoethanol protects against thermal inactivation | Herpes simplex virus |
| 40 | - |
the half-life of 3 min increases to 6 min in presence of 2 mM ATP or to 30 min in presence of 0.12 mM thymidine | Herpes simplex virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Herpes simplex virus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | enzyme is active between pH 6 and pH 10 | Herpes simplex virus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
iododeoxycytidine | pH 7.8, 37°C | Herpes simplex virus | |
| 0.3 | - |
acyclovir | pH 7.8, 37°C | Herpes simplex virus | |
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