Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.21 extracted from

  • Bresnick, E.
    Deoxythymidine kinase in regenerating rat liver (1978), Methods Enzymol., 51, 360-365.
    View publication on PubMed
Search for more literature for 2.7.1.21

Inhibitors

Inhibitors Comment Organism Structure
5-bromodeoxyuridine competitive to thymidine Rattus norvegicus
5-Chlorodeoxyuridine competitive to thymidine Rattus norvegicus
5-fluorodeoxyuridine competitive to thymidine Rattus norvegicus
5-iododeoxyuridine competitive to thymidine Rattus norvegicus
5-Trifluoromethyldeoxyuridine competitive to thymidine Rattus norvegicus
Ba2+
-
 Rattus norvegicus
Be2+
-
 Rattus norvegicus
Ca2+
-
 Rattus norvegicus
Co2+
-
 Rattus norvegicus
deoxyuridine competitive to thymidine Rattus norvegicus
dTTP non-competitive Rattus norvegicus
Fe2+
-
 Rattus norvegicus
Mn2+
-
 Rattus norvegicus
Ni2+
-
 Rattus norvegicus
Sr2+
-
 Rattus norvegicus
Zn2+
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0056
-
 thymidine pH 8.0, 37°C, Km is markedly dependent on pH Rattus norvegicus
0.8
-
 MgCl2 pH 8 Rattus norvegicus
2.6
-
 ATP at concentrations above 1 mM, pH 8, 37°C Rattus norvegicus
20
-
 ATP at concentrations below 1 mM, pH 8, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can partially substitute for Mg2+ Rattus norvegicus
Co2+ can partially substitute for Mg2+ Rattus norvegicus
Mg2+ Km: 0.8 mM Rattus norvegicus
Mn2+ can partially substitute for Mg2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81000
-
 sucrose density gradient centrifugation Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxyuridine
-
 Rattus norvegicus ADP + 2'-deoxyuridine 5'-phosphate
-
 ?
ATP + 5-bromo-2'-deoxyuridine
-
 Rattus norvegicus ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
-
 ?
ATP + 5-chloro-2'-deoxyuridine
-
 Rattus norvegicus ADP + 5-chloro-2'-deoxyuridine 5'-phosphate
-
 ?
ATP + 5-fluoro-2'-deoxyuridine
-
 Rattus norvegicus ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
 ?
ATP + 5-iodo-2'-deoxyuridine
-
 Rattus norvegicus ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
-
 ?
ATP + 5-trifluoromethyl-2'-deoxyuridine
-
 Rattus norvegicus ADP + 5-trifluoromethyl-2'-deoxyuridine 5'-phosphate
-
 ?
ATP + 6-aza-2'-deoxythymidine
-
 Rattus norvegicus ADP + 6-aza-2'-deoxythymidine 5'-phosphate
-
 ?
ATP + thymidine
-
 Rattus norvegicus ADP + dTMP
-
 ?
additional information 5-halogenated cytidines, bromouridine, iodouridine, uridine, 5-fluorodeoxycytidine, purine ribonucleosides Rattus norvegicus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.001
-
 Be2+ pH 8, 37°C Rattus norvegicus
0.022
-
 5-fluorodeoxyuridine pH 8, 37°C Rattus norvegicus
0.11
-
 deoxyuridine pH 8, 37°C Rattus norvegicus