Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dCTP | activation | Escherichia coli | |
GTP | activation | Escherichia coli | |
Hydroxymethyl-dCDP | activation | Escherichia coli | |
Hydroxymethyl-dCTP | activation | Escherichia coli | |
additional information | Km values of allosteric regulators | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-N-methyl-5-iodo-2'-deoxyuridine | - |
Escherichia coli | |
dTTP | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
5-iodo-2'-deoxyuridine | - |
Escherichia coli | |
0.0017 | - |
deoxythymidine | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli | |
Mn2+ | can partially substitute for Mg2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
720 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C, 10 mM 2-mercaptoethanol, 0.5 mg/ml bovine serum albumin, several months | Escherichia coli |
0°C, 2-3 months, change of kinetic properties, larger amount of ATP is required to get the same rate of reaction | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-bromo-2'-deoxyuridine | ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP | Escherichia coli | ADP + 5-bromo-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + 5-chloro-2'-deoxyuridine | ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP | Escherichia coli | ADP + 5-chloro-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + 5-fluoro-2'-deoxyuridine | ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP | Escherichia coli | ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + 5-iodo-2'-deoxyuridine | ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP | Escherichia coli | ADP + 5-iodo-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + 5-mercapto-2'-deoxyuridine | ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP | Escherichia coli | ADP + 5-mercapto-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + deoxythymidine | ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP | Escherichia coli | ADP + dTMP | - |
? | |
ATP + deoxyuridine | ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP | Escherichia coli | ADP + dUMP | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dATP | activation | Escherichia coli | |
dGTP | activation | Escherichia coli | |
GDP | activation | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
3-N-methyl-5-iodo-2'-deoxyuridine | pH 7.8, 37°C | Escherichia coli |