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Literature summary for 2.7.1.21 extracted from
- Thymidine kinase from Escherichia coli (1978), Methods Enzymol., 51, 354-360.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dCTP | activation | Escherichia coli |  |
| GTP | activation | Escherichia coli | |
| Hydroxymethyl-dCDP | activation | Escherichia coli | |
| Hydroxymethyl-dCTP | activation | Escherichia coli | |
| additional information | Km values of allosteric regulators | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-N-methyl-5-iodo-2'-deoxyuridine | - |
Escherichia coli | |
| dTTP | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
5-iodo-2'-deoxyuridine | - |
Escherichia coli | |
| 0.0017 | - |
deoxythymidine | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli | |
| Mn2+ | can partially substitute for Mg2+ | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 720 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 10 mM 2-mercaptoethanol, 0.5 mg/ml bovine serum albumin, several months | Escherichia coli |
| 0°C, 2-3 months, change of kinetic properties, larger amount of ATP is required to get the same rate of reaction | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-bromo-2'-deoxyuridine | ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP | Escherichia coli | ADP + 5-bromo-2'-deoxyuridine 5'-phosphate | - |
? | |
| ATP + 5-chloro-2'-deoxyuridine | ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP | Escherichia coli | ADP + 5-chloro-2'-deoxyuridine 5'-phosphate | - |
? | |
| ATP + 5-fluoro-2'-deoxyuridine | ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP | Escherichia coli | ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate | - |
? | |
| ATP + 5-iodo-2'-deoxyuridine | ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP | Escherichia coli | ADP + 5-iodo-2'-deoxyuridine 5'-phosphate | - |
? | |
| ATP + 5-mercapto-2'-deoxyuridine | ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP | Escherichia coli | ADP + 5-mercapto-2'-deoxyuridine 5'-phosphate | - |
? | |
| ATP + deoxythymidine | ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP | Escherichia coli | ADP + dTMP | - |
? | |
| ATP + deoxyuridine | ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP | Escherichia coli | ADP + dUMP | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| dATP | activation | Escherichia coli | |
| dGTP | activation | Escherichia coli | |
| GDP | activation | Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
3-N-methyl-5-iodo-2'-deoxyuridine | pH 7.8, 37°C | Escherichia coli | |
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