Cloned (Comment) | Organism |
---|---|
CpAK gene codon-optimized for expression in Escherichia coli | Cryptosporidium parvum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-chloroadenosine | adenosine phosphorylation is inhibited by 100fold excess of the inhibitor | Cryptosporidium parvum | |
4-nitro-6-benzylthioguanosine | 0.2 mM, partial inhibition | Cryptosporidium parvum | |
4-nitro-6-benzylthioinosine | 0.2 mM, partial inhibition | Cryptosporidium parvum | |
5'-amino-5'-deoxyadenosine | adenosine phosphorylation is inhibited by 100fold excess of the inhibitor | Cryptosporidium parvum | |
5'-deoxyadenosine | adenosine phosphorylation is inhibited by 100fold excess of the inhibitor | Cryptosporidium parvum | |
adenosine | strongly inhibited by 100fold excess adenosine | Cryptosporidium parvum | |
EDTA | 100 mM, complete inhibition | Cryptosporidium parvum | |
tubercidin | adenosine phosphorylation is inhibited by 100fold excess of the inhibitor | Cryptosporidium parvum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
adenosine | 37°C, pH 8.0 | Cryptosporidium parvum | |
0.041 | - |
ATP | 37°C, pH 8.0 | Cryptosporidium parvum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) | Cryptosporidium parvum | |
Co2+ | activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) | Cryptosporidium parvum | |
Fe2+ | activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) | Cryptosporidium parvum | |
Mg2+ | activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) | Cryptosporidium parvum | |
Mn2+ | activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) | Cryptosporidium parvum | |
Zn2+ | activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) | Cryptosporidium parvum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptosporidium parvum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Cryptosporidium parvum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine | no phosphorylation of inosine or guanosine | Cryptosporidium parvum | ADP + AMP | - |
? | |
CTP + adenosine | about 60% of activity with ATP | Cryptosporidium parvum | CDP + AMP | - |
? | |
GTP + adenosine | about 50% of activity with ATP | Cryptosporidium parvum | GDP + AMP | - |
? | |
TTP + adenosine | about 10% of activity with ATP | Cryptosporidium parvum | TDP + AMP | - |
? | |
UTP + adenosine | about 40% of activity with ATP | Cryptosporidium parvum | UDP + AMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CpAK | - |
Cryptosporidium parvum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
ATP | 37°C, pH 8.0 | Cryptosporidium parvum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Cryptosporidium parvum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.5 | pH 5.5: about 40% of maximal activity, pH 10.5: about 65% of maximal activity | Cryptosporidium parvum |