Literature summary for 2.7.1.20 extracted from

Wang, H.; Hao, L.; Shung, C.Y.; Sunter, G.; Bisaro, D.M.
Adenosine kinase is inactivated by geminivirus AL2 and L2 proteins (2003), Plant Cell, 15, 3020-3032.

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	the enzyme activity is increased in plants after inoculation with diverse RNA viruses or a germinivirus lacking an intact L2 protein	Nicotiana benthamiana

Cloned(Commentary)

Cloned (Comment)	Organism
DNA sequence determination and analysis, the His-tagged enzyme is expressed with or without coexpression of viral AL2 and L2 proteins in an enzyme-deficient Escherichia coli strain, enzyme expression leads to complementation of the mutant, coexpression leads to inactivation of the enzyme and no complementation, expression in yeast cells utilizing the two-hybrid system coexpressing the L2 protein of geminivirus, coexpression leads to inactivation of the enzyme	Arabidopsis thaliana
expression of viral proteins L2 and AL2 in nucelus and cytosol of transgenic plants via the Agrobacterium infection system highly reduces enzyme activity	Nicotiana benthamiana

Cloned (Comment)

Organism

DNA sequence determination and analysis, the His-tagged enzyme is expressed with or without coexpression of viral AL2 and L2 proteins in an enzyme-deficient Escherichia coli strain, enzyme expression leads to complementation of the mutant, coexpression leads to inactivation of the enzyme and no complementation, expression in yeast cells utilizing the two-hybrid system coexpressing the L2 protein of geminivirus, coexpression leads to inactivation of the enzyme

Arabidopsis thaliana

expression of viral proteins L2 and AL2 in nucelus and cytosol of transgenic plants via the Agrobacterium infection system highly reduces enzyme activity

Nicotiana benthamiana

Inhibitors

Inhibitors	Comment	Organism
EDTA	-	Arabidopsis thaliana
geminivirus AL2	inactivation of the enzyme in vitro and in vivo via direct interaction, AL2 is a RNA geminivirus of the Begomovirus genera encoded protein serving as pathogenicity determinant causing enhanced susceptibility of target organisms, overview	Arabidopsis thaliana
geminivirus AL2	inactivation of the enzyme in vitro and in vivo via direct interaction, AL2 is a RNA geminivirus of the Begomovirus genera encoded protein serving as pathogenicity determinant causing enhanced susceptibility of target organisms, overview	Nicotiana benthamiana
geminivirus L2	inactivation of the enzyme in vitro and in vivo via direct interaction, L2 is a RNA geminivirus of the Curtovirus genera encoded protein serving as pathogenicity determinant causing enhanced susceptibility of target organisms, overview	Arabidopsis thaliana
geminivirus L2	inactivation of the enzyme in vitro and in vivo via direct interaction, L2 is a RNA geminivirus of the Curtovirus genera encoded protein serving as pathogenicity determinant causing enhanced susceptibility of target organisms, overview	Nicotiana benthamiana

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Nicotiana benthamiana
Mg2+	-	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + adenosine	Nicotiana benthamiana	the enzyme catalyzes the salvage synthesis of 5'-AMP from ADP and ATP	ADP + AMP	-	?
ATP + adenosine	Arabidopsis thaliana	the enzyme catalyzes the salvage synthesis of 5'-AMP from ADP and ATP	ADP + AMP	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9LZG0	2 isozymes ADK1 and ADK2	-
Nicotiana benthamiana	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partially, recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + adenosine	-	Nicotiana benthamiana	ADP + AMP	-	?
ATP + adenosine	-	Arabidopsis thaliana	ADP + AMP	-	?
ATP + adenosine	the enzyme catalyzes the salvage synthesis of 5'-AMP from ADP and ATP	Nicotiana benthamiana	ADP + AMP	-	?
ATP + adenosine	the enzyme catalyzes the salvage synthesis of 5'-AMP from ADP and ATP	Arabidopsis thaliana	ADP + AMP	-	?

Synonyms

Synonyms	Comment	Organism
ADK	-	Nicotiana benthamiana
ADK	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Nicotiana benthamiana
37	-	assay at	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	assay at	Nicotiana benthamiana
7.6	-	assay at	Arabidopsis thaliana