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Literature summary for 2.7.1.20 extracted from

  • Wu, J.Z.; Larson, G.; Walker, H.; Shim, J.H.; Hong, Z.
    Phosphorylation of ribavirin and viramidine by adenosine kinase and cytosolic 5'-nucleotidase II: Implications for ribavirin metabolism in erythrocytes (2005), Antimicrob. Agents Chemother., 49, 2164-2171.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.20

Activating Compound

Activating Compound Comment Organism Structure
additional information no inhibition by phosphate, the activity in higher in phosphate buffer compared to HEPES buffer Cricetulus griseus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cricetulus griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0032
-
 adenosine recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline Cricetulus griseus
0.54
-
 ribavirin recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline Cricetulus griseus
7.7
-
 viramidine recombinant enzyme, pH 9.0, 37°C, phosphate buffered saline Cricetulus griseus
7.8
-
 ribavirin recombinant enzyme, pH 7.3, 37°C, 0.1 M HEPES buffer Cricetulus griseus
16
-
 viramidine recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline Cricetulus griseus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ at 1.5 mM MgCl2 Cricetulus griseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + adenosine Cricetulus griseus
-
 ADP + AMP
-
 ?

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli to homogeneity Cricetulus griseus

Source Tissue

Source Tissue Comment Organism Textmining
CHO cell
-
 Cricetulus griseus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Cricetulus griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine
-
 Cricetulus griseus ADP + AMP
-
 ?
ATP + ribavirin very low activity Cricetulus griseus ADP + ribavirin 5'-phosphate
-
 ?
ATP + viramidine very low activity Cricetulus griseus ADP + viramidine 5'-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Cricetulus griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.16
-
 viramidine recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline Cricetulus griseus
0.26
-
 viramidine recombinant enzyme, pH 9.0, 37°C, phosphate buffered saline Cricetulus griseus
0.62
-
 ribavirin recombinant enzyme, pH 7.3, 37°C, 0.1 M HEPES buffer Cricetulus griseus
1.8
-
 ribavirin recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline Cricetulus griseus
13
-
 adenosine recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline Cricetulus griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 assay at Cricetulus griseus