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Literature summary for 2.7.1.20 extracted from

  • Xiao-Bing, L.V.; Hai-Zhen, W.U.; Jiang, Y.E.; Yi, F.; Hui-Zhan, Z.
    Expression, purification and characterization of recombinant Saccharomyces cerevisiae adenosine kinase (2003), Acta Biochim. Biophys. Sin., 35, 666-670.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
adenosine pH 8.0, 37ºC Saccharomyces cerevisiae
0.1
-
ATP pH 8.0, 37ºC Saccharomyces cerevisiae
0.66
-
2'-deoxyadenosine pH 8.0, 37ºC Saccharomyces cerevisiae
0.8
-
Inosine pH 8.0, 37ºC Saccharomyces cerevisiae
0.86
-
uridine pH 8.0, 37ºC Saccharomyces cerevisiae
0.9
-
cytidine pH 8.0, 37ºC Saccharomyces cerevisiae
2.24
-
guanosine pH 8.0, 37ºC Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36400
-
predicted from amino acid sequence Saccharomyces cerevisiae
37000
-
1 * 37000, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation and chromatography on DEAE-Sepharose, 20fold purification Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20ºC, 10% glycerol, purified preparation Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxy-adenosine
-
Saccharomyces cerevisiae ADP + 2'-deoxy-AMP
-
?
ATP + adenosine
-
Saccharomyces cerevisiae ADP + AMP
-
?
ATP + cytidine
-
Saccharomyces cerevisiae ?
-
?
ATP + guanosine
-
Saccharomyces cerevisiae ?
-
?
ATP + inosine
-
Saccharomyces cerevisiae ADP + inosine 5'-phosphate
-
?
ATP + uridine
-
Saccharomyces cerevisiae ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 37000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
guanosine 51fold reduction Saccharomyces cerevisiae
0.583
-
Inosine 44fold reduction Saccharomyces cerevisiae
1
-
2'-deoxyadenosine 25fold reduction Saccharomyces cerevisiae
1.02
-
cytidine 25fold reduction Saccharomyces cerevisiae
1.67
-
uridine 15fold reduction Saccharomyces cerevisiae
25.5
-
adenosine
-
Saccharomyces cerevisiae