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Literature summary for 2.7.1.20 extracted from

  • Faye, F.; Le Floc'h, F.
    Adenosine kinase of peach tree flower buds: purification and properties (1997), Plant Physiol. Biochem., 35, 15-22.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxyadenosine no inhibition Prunus persica
3'-deoxyadenosine no inhibition Prunus persica
ADP non competitive with respect to adenosine and competitive with respect to ATP; product inhibition Prunus persica
AMP mixed-type inhibition towards adenosine and ATP; product inhibition Prunus persica
GDP non competitive with respect to adenosine and competitive with respect to ATP Prunus persica
N6-delta2-isopentenyl-adenosine competitive with respect to adenosine Prunus persica
NH4+ slight inhibition Prunus persica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018 0.006 adenosine pH 9, 25ºC Prunus persica
0.2 0.7 ATP pH 9, 25ºC Prunus persica

Metals/Ions

Metals/Ions Comment Organism Structure
KCl strong activation, maximum activity at 100 mM Prunus persica
Mg2+ 0.3-0.4 mM, in the presence of K+ Prunus persica
Mn2+ at 0.4-0.5 mM, in the presence of K+ Prunus persica
Rb+ activation, at 100 mM, weaker than K+ Prunus persica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
gel filtration Prunus persica
434000
-
1 * 434000, SDS-PAGE Prunus persica

Organism

Organism UniProt Comment Textmining
Prunus persica
-
Batsch cv Redhaven, peach
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, gel filtration ion exchange and affinity chromatography Prunus persica

Source Tissue

Source Tissue Comment Organism Textmining
flower buds Prunus persica
-

Storage Stability

Storage Stability Organism
-25ºC, variable losses of activity Prunus persica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine best substrates Prunus persica ADP + AMP
-
?
CTP + adenosine
-
Prunus persica CDP + AMP
-
?
GTP + adenosine less efficient than ATP Prunus persica GDP + AMP
-
?
UTP + adenosine
-
Prunus persica UDP + AMP
-
?

Subunits

Subunits Comment Organism
monomer 1 * 434000, SDS-PAGE Prunus persica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9.5
-
Prunus persica

pH Range

pH Minimum pH Maximum Comment Organism
8.5 9.5 sharp drop of activity outside the range, especially between pH 6.5 and pH 8.5 Prunus persica