Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-deoxyadenosine | no inhibition | Prunus persica | |
3'-deoxyadenosine | no inhibition | Prunus persica | |
ADP | non competitive with respect to adenosine and competitive with respect to ATP; product inhibition | Prunus persica | |
AMP | mixed-type inhibition towards adenosine and ATP; product inhibition | Prunus persica | |
GDP | non competitive with respect to adenosine and competitive with respect to ATP | Prunus persica | |
N6-delta2-isopentenyl-adenosine | competitive with respect to adenosine | Prunus persica | |
NH4+ | slight inhibition | Prunus persica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | 0.006 | adenosine | pH 9, 25ºC | Prunus persica | |
0.2 | 0.7 | ATP | pH 9, 25ºC | Prunus persica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | strong activation, maximum activity at 100 mM | Prunus persica | |
Mg2+ | 0.3-0.4 mM, in the presence of K+ | Prunus persica | |
Mn2+ | at 0.4-0.5 mM, in the presence of K+ | Prunus persica | |
Rb+ | activation, at 100 mM, weaker than K+ | Prunus persica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Prunus persica |
434000 | - |
1 * 434000, SDS-PAGE | Prunus persica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prunus persica | - |
Batsch cv Redhaven, peach | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, gel filtration ion exchange and affinity chromatography | Prunus persica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | buds | Prunus persica | - |
Storage Stability | Organism |
---|---|
-25ºC, variable losses of activity | Prunus persica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine | best substrates | Prunus persica | ADP + AMP | - |
? | |
CTP + adenosine | - |
Prunus persica | CDP + AMP | - |
? | |
GTP + adenosine | less efficient than ATP | Prunus persica | GDP + AMP | - |
? | |
UTP + adenosine | - |
Prunus persica | UDP + AMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 434000, SDS-PAGE | Prunus persica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | - |
Prunus persica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | sharp drop of activity outside the range, especially between pH 6.5 and pH 8.5 | Prunus persica |