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Literature summary for 2.7.1.20 extracted from

  • Recacha, R.; Talalaev, A.; DeLucas, L.J.; Chattopadhyay, D.
    Toxoplasma gondii adenosine kinase: expression, purification, characterization, crystallization and preliminary crystallographic analysis (2000), Acta Crystallogr. Sect. D, 56, 76-78.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Toxoplasma gondii

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor-diffusion Toxoplasma gondii

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Toxoplasma gondii
Hg2+ strong inhibition, reducing agent reverses Toxoplasma gondii
additional information not influenced by DTT Toxoplasma gondii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Toxoplasma gondii
Mg2+
-
Toxoplasma gondii
Mn2+
-
Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38200
-
gel filtration Toxoplasma gondii
40000
-
1 * 40000, SDS-PAGE Toxoplasma gondii

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ion exchange Toxoplasma gondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine
-
Toxoplasma gondii ADP + AMP
-
?
GTP + adenosine less efficient than ATP Toxoplasma gondii GDP + AMP
-
?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Toxoplasma gondii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Toxoplasma gondii

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
Toxoplasma gondii