Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine | no inhibition; strong, with formycin A as substrate | Leishmania donovani | |
Formycin A | at very high concentrations, kinetics | Leishmania donovani | |
NEM | moderate inhibition; strong inhibition with formycin A as substrate, adenosine protects | Leishmania donovani | |
Polyclonal antibodies to adenosine kinase from Leishmania donovani | not from other eukaryotic sources | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
adenosine | - |
Leishmania donovani | |
0.016 | - |
adenosine | pH 7.5, 30ºC | Leishmania donovani | |
0.02 | 0.024 | ATP | formycin A | Leishmania donovani | |
0.02 | 0.024 | ATP | ATP in form of MgATP2- | Leishmania donovani | |
0.024 | - |
Formycin A | pH 7.5, 30ºC | Leishmania donovani |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | activation, formycin A as substrate. Adenosine phosphorylation is neither stimulated nor inhibited | Leishmania donovani | |
Mg2+ | requirement | Leishmania donovani | |
Mg2+ | reactive species: MgATP2- | Leishmania donovani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine | - |
Leishmania donovani | ADP + AMP | - |
? | |
ATP + formycin A | - |
Leishmania donovani | ADP + formycin A 5'-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Leishmania donovani |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24 | - |
adenosine | formycin A | Leishmania donovani |