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Literature summary for 2.7.1.20 extracted from
- Adenosine kinase from rabbit liver. I. Purification by affinity chromatography and properties (1979), J. Biol. Chem., 254, 2339-2345.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine | kinetics | Oryctolagus cuniculus |  |
| ATP | - |
Oryctolagus cuniculus | |
| EDTA | at high concentrations | Oryctolagus cuniculus | |
| Mg2+ | - |
Oryctolagus cuniculus | |
| additional information | no inhibition by PMSF, EGTA | Oryctolagus cuniculus | |
| p-hydroxymercuribenzoate | DTT reverses | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00015 | 0.0005 | adenosine | pH 6.8, 30ºC | Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Oryctolagus cuniculus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | no activation | Oryctolagus cuniculus | |
| Ca2+ | activation | Oryctolagus cuniculus | |
| Mg2+ | requirement | Oryctolagus cuniculus | |
| Mn2+ | can partially substitute for Mg2+ | Oryctolagus cuniculus | |
| Mn2+ | activation | Oryctolagus cuniculus | |
| additional information | emission spectral analysis show that enzyme contains tightly bound divalent metal ions | Oryctolagus cuniculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34500 | - |
gel filtration | Oryctolagus cuniculus |
| 51000 | - |
1 * 51000, SDS-PAGE | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + adenosine | Oryctolagus cuniculus | involved in phosphorylation of many pharmacological active purine nucleosides | ADP + AMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration and affinity chromatography | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.75 | 2.23 | - |
Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + adenosine | specificity | Oryctolagus cuniculus | ADP + AMP | - |
? | |
| ATP + adenosine | best substrates | Oryctolagus cuniculus | ADP + AMP | - |
? | |
| ATP + adenosine | ATP-analogues are also substrates | Oryctolagus cuniculus | ADP + AMP | - |
? | |
| ATP + adenosine | involved in phosphorylation of many pharmacological active purine nucleosides | Oryctolagus cuniculus | ADP + AMP | - |
? | |
| CTP + adenosine | CTP is a poor substrate | Oryctolagus cuniculus | CDP + AMP | - |
? | |
| GTP + adenosine | - |
Oryctolagus cuniculus | GDP + AMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 51000, SDS-PAGE | Oryctolagus cuniculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the pH-optimum is a function of the adenosine concentration | Oryctolagus cuniculus |
| additional information | - |
the pH-optimum is a function of the ATP/Mg2+ ratio | Oryctolagus cuniculus |
| 4.8 | - |
below, ATP/Mg2+ ratio: 0.1 | Oryctolagus cuniculus |
| 5.2 | - |
ATP/Mg2+ ratio: 1 | Oryctolagus cuniculus |
| 5.5 | - |
ATP/Mg2+ ratio: 10 | Oryctolagus cuniculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | 5.8 | about half-maximal activity at pH 4.6 and pH 5.8, ATP/Mg2+ ratio: 1 | Oryctolagus cuniculus |
| 5.2 | 6.4 | about half-maximal activity at pH 5.2 and pH 6.4, ATP/Mg2+ ratio: 10 | Oryctolagus cuniculus |
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