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Literature summary for 2.7.1.20 extracted from

  • Hawkins, C.F.; Bagnara, A.S.
    Adenosine kinase from human erythrocytes: kinetic studies and characterization of adenosine binding sites (1987), Biochemistry, 26, 1982-1987.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
AMP activation below 5 mM, inhibits above 5 mM Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
adenosine pH and Mg2+ dependent, most severe at pH 7.4, less inhibition at pH 8.0 and no inhibition at pH 6.5 Homo sapiens
AMP product inhibition, above 5 mM Homo sapiens
DTNB substrates protect Homo sapiens
MgADP- product inhibition, linear, noncompetitive with respect to MgATP2- and adenosine Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
chromatography on CM-cellulose, chromatography on DEAE-Sephacel and affinity chromatography Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP + adenosine = ADP + AMP mechanism Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine
-
Homo sapiens ADP + AMP
-
?
GTP + adenosine best substrate Homo sapiens GDP + AMP
-
?
GTP + adenosine more effective than ATP Homo sapiens GDP + AMP
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0059 0.045 adenosine pH 7.4, 37ºC, at variable AMP and Mg2+ concentrations Homo sapiens
0.03 0.534 MgADP- pH 8.0, 37ºC, at variable MgATP2- concentrations Homo sapiens
0.051 0.238 adenosine pH 8.0, 37ºC, at variable AMP and Mg2+ concentrations Homo sapiens
0.362 0.428 MgADP- pH 8.0, 37ºC, at variable adenosine concentrations Homo sapiens
1.84
-
AMP pH 8.0, 37ºC, at variable adenosine concentrations Homo sapiens