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Literature summary for 2.7.1.2 extracted from
- Enhancement of gene expression in Escherichia coli and characterization of highly stable ATP-dependent glucokinase from Pyrobaculum calidifontis (2018), Extremophiles, 22, 247-257 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli. Expression of the gene is low resulting in a poor yield of the recombinant protein. A single site directed mutation in Pcal_1032 gene (Nucleotide G at position 11 is replaced by A), without altering the amino acid sequence, results in approximately tenfold higher expression | Pyrobaculum calidifontis |
General Stability
| General Stability | Organism |
|---|---|
| highly stable in the presence of denaturants | Pyrobaculum calidifontis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.66 | - |
D-glucose | pH 8.5, 50°C | Pyrobaculum calidifontis |  |
| 0.9 | - |
ATP | pH 8.5, 50°C | Pyrobaculum calidifontis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
gel filtration | Pyrobaculum calidifontis |
| 31573 | - |
calculated from sequence | Pyrobaculum calidifontis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | Pyrobaculum calidifontis | - |
ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | Pyrobaculum calidifontis JCM 11548 | - |
ADP + D-glucose 6-phosphate | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| guanidine-HCl | no significant change in the CD spectra and enzyme activity after overnight incubation in the presence of 8 M urea or 1 M guanidinium chloride | Pyrobaculum calidifontis |
| urea | no significant change in the CD spectra and enzyme activity after overnight incubation in the presence of 8 M urea or 1 M guanidinium chloride | Pyrobaculum calidifontis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrobaculum calidifontis | A3MUZ0 | - |
- |
| Pyrobaculum calidifontis JCM 11548 | A3MUZ0 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | at 70% of the activity as compared to D-glucose | Pyrobaculum calidifontis | ADP + D-fructose 6-phosphate | - |
? | |
| ATP + D-fructose | at 70% of the activity as compared to D-glucose | Pyrobaculum calidifontis JCM 11548 | ADP + D-fructose 6-phosphate | - |
? | |
| ATP + D-galactose | at 11% of the activity as compared to D-glucose | Pyrobaculum calidifontis | ADP + D-galactose | - |
? | |
| ATP + D-galactose | at 11% of the activity as compared to D-glucose | Pyrobaculum calidifontis JCM 11548 | ADP + D-galactose | - |
? | |
| ATP + D-glucose | - |
Pyrobaculum calidifontis | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | the enzyme phosphorylates various hexoses with a marked preference for glucose. ATP is the most preferred phosphoryl group donor. No activity with ADP or AMP as phosphoryl group donor | Pyrobaculum calidifontis | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | - |
Pyrobaculum calidifontis JCM 11548 | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | the enzyme phosphorylates various hexoses with a marked preference for glucose. ATP is the most preferred phosphoryl group donor. No activity with ADP or AMP as phosphoryl group donor | Pyrobaculum calidifontis JCM 11548 | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-mannose | at 30% of the activity as compared to D-glucose | Pyrobaculum calidifontis | ADP + D-mannose 6-phosphate | - |
? | |
| ATP + D-mannose | at 30% of the activity as compared to D-glucose | Pyrobaculum calidifontis JCM 11548 | ADP + D-mannose 6-phosphate | - |
? | |
| ATP + D-sorbitol | at 10% of the activity as compared to D-glucose | Pyrobaculum calidifontis | ADP + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 31573, calculated from sequence | Pyrobaculum calidifontis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
- |
Pyrobaculum calidifontis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
half-life: 25 min | Pyrobaculum calidifontis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 289 | - |
D-glucose | pH 8.5, 50°C | Pyrobaculum calidifontis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pyrobaculum calidifontis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pyrobaculum calidifontis | calculated from sequence | - |
4.64 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 437 | - |
D-glucose | pH 8.5, 50°C | Pyrobaculum calidifontis | |
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