Cloned (Comment) | Organism |
---|---|
heterologous expression in Escherichia coli BL21 (DE3) as a C-terminal His6 version in an IPTG-inducible expression vector | Methylotuvimicrobium alcaliphilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Methylotuvimicrobium alcaliphilum | |
KCl | 0.2 M, 50% loss of activity | Methylotuvimicrobium alcaliphilum | |
additional information | not affected by glucose 6-phosphate | Methylotuvimicrobium alcaliphilum | |
NaCl | 0.8 M, 50% loss of activity | Methylotuvimicrobium alcaliphilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
D-glucose | pH 9.5, 30°C | Methylotuvimicrobium alcaliphilum | |
0.37 | - |
ATP | pH 9.5, 30°C | Methylotuvimicrobium alcaliphilum | |
1.1 | - |
D-glucosamine | pH 9.5, 30°C | Methylotuvimicrobium alcaliphilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme is Mg2+- or Mn2+-dependent. KM: 0.38 mM | Methylotuvimicrobium alcaliphilum | |
Mn2+ | the enzyme is Mg2+- or Mn2+-dependent. KM: 0.4 mM | Methylotuvimicrobium alcaliphilum | |
additional information | CuCl2, CoCl2, ZnCl2 or CaCl2 have no appreciable effect on the enzyme activity | Methylotuvimicrobium alcaliphilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gradient electrophoresis | Methylotuvimicrobium alcaliphilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | Methylotuvimicrobium alcaliphilum | - |
ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | Methylotuvimicrobium alcaliphilum 20Z | - |
ADP + D-glucose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylotuvimicrobium alcaliphilum | G4T1H4 | - |
- |
Methylotuvimicrobium alcaliphilum 20Z | G4T1H4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methylotuvimicrobium alcaliphilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucosamine | D-glucose and D-glucosamine with nearly equal activity | Methylotuvimicrobium alcaliphilum | ADP + D-glucosamine 6-phosphate | - |
? | |
ATP + D-glucosamine | D-glucose and D-glucosamine with nearly equal activity | Methylotuvimicrobium alcaliphilum 20Z | ADP + D-glucosamine 6-phosphate | - |
? | |
ATP + D-glucose | - |
Methylotuvimicrobium alcaliphilum | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | D-glucose and D-glucosamine with nearly equal activity | Methylotuvimicrobium alcaliphilum | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | - |
Methylotuvimicrobium alcaliphilum 20Z | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | D-glucose and D-glucosamine with nearly equal activity | Methylotuvimicrobium alcaliphilum 20Z | ADP + D-glucose 6-phosphate | - |
? | |
additional information | fructose, xylose, galactose, sucrose, lactose, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate or fructose-6-phosphate as a phosphate acceptor. GTP, CTP, UTP, ADP or PPi does not serve as a phosphoryl donor | Methylotuvimicrobium alcaliphilum | ? | - |
- |
|
additional information | fructose, xylose, galactose, sucrose, lactose, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate or fructose-6-phosphate as a phosphate acceptor. GTP, CTP, UTP, ADP or PPi does not serve as a phosphoryl donor | Methylotuvimicrobium alcaliphilum 20Z | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 35000, SDS-PAGE | Methylotuvimicrobium alcaliphilum |
Synonyms | Comment | Organism |
---|---|---|
glk | - |
Methylotuvimicrobium alcaliphilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Methylotuvimicrobium alcaliphilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
252.8 | - |
ATP | pH 9.5, 30°C | Methylotuvimicrobium alcaliphilum | |
257.7 | - |
D-glucose | pH 9.5, 30°C | Methylotuvimicrobium alcaliphilum | |
293.5 | - |
D-glucosamine | pH 9.5, 30°C | Methylotuvimicrobium alcaliphilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Methylotuvimicrobium alcaliphilum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.34 | - |
ADP | pH 9.5, 30°C | Methylotuvimicrobium alcaliphilum |
General Information | Comment | Organism |
---|---|---|
malfunction | chromosomal deletion of the glk gene resulted in a loss of glucokinase activity and retardation of growth as well as in a decrease of intracellular glycogen content | Methylotuvimicrobium alcaliphilum |
metabolism | the enzyme implicated in the regulation of glycogen biosynthesis and degradation in an obligate methanotroph | Methylotuvimicrobium alcaliphilum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
266.8 | - |
D-glucosamine | pH 9.5, 30°C | Methylotuvimicrobium alcaliphilum | |
683.2 | - |
ATP | pH 9.5, 30°C | Methylotuvimicrobium alcaliphilum | |
805.3 | - |
D-glucose | pH 9.5, 30°C | Methylotuvimicrobium alcaliphilum |