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Literature summary for 2.7.1.2 extracted from

  • Cordeiro, A.T.; Caceres, A.J.; Vertommen, D.; Concepcion, J.L.; Michels, P.A.; Versees, W.
    The crystal structure of Trypanosoma cruzi glucokinase reveals features determining oligomerization and anomer specificity of hexose-phosphorylating enzymes (2007), J. Mol. Biol., 372, 1215-1226.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of TcGlcK in complex with D-glucose and ADP are obtained by the hanging-drop, vapor-diffusion method, using PEG3350 as precipitant agent and diammonium hydrogen citrate as additive. A complete native dataset is collected to 2.1 A maximum resolution Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic constants of TcGlcK obtained for different anomer solutions of D-glucose Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q4E4E1
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged recombinant TcGlcK is expressed in the Escherichia coli BL21 strain and purified to homogeneity by nickel affinity chromatography Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
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Trypanosoma cruzi ADP + D-glucose 6-phosphate
-
?

Synonyms

Synonyms Comment Organism
TcGlcK
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Trypanosoma cruzi