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Literature summary for 2.7.1.2 extracted from
- ATP-dependent glucokinase from the hyperthermophilic bacterium Thermotoga maritima represents an extremely thermophilic ROK glucokinase with high substrate specificity (2003), FEMS Microbiol. Lett., 226, 405-411.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene TM 1469 or glk, functional expression of the soluble His-tagged in Escherichia coli strain BL21(DE3) | Thermotoga maritima |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Thermotoga maritima |  |
| Fe2+ | - |
Thermotoga maritima | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | activation energy, kinetics | Thermotoga maritima | |
| 0.36 | - |
ATP | recombinant enyme, pH 7.3, 80°C | Thermotoga maritima | |
| 1 | - |
D-glucose | recombinant enyme, pH 7.3, 80°C | Thermotoga maritima | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 50% of the activity with Mg2+ | Thermotoga maritima | |
| Mg2+ | most efficient divalent cation | Thermotoga maritima | |
| Mn2+ | 90% of the activity with Mg2+ | Thermotoga maritima | |
| Ni2+ | 20% of the activity with Mg2+ | Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
2 * 36000, alpha2, recombinant enzyme, SDS-PAGE | Thermotoga maritima |
| 75000 | - |
recombinant enzyme, gel filtration | Thermotoga maritima |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | Thermotoga maritima | the enzyme catalyzes the first step of the Embden-Meyerhoff pathway and the Entner-Douderoff pathway | ADP + D-glucose 6-phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
gene TM 1469 or glk | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3) by ultracentrifugation, nickel affinity chromatography, and gel filtration to homogeneity | Thermotoga maritima |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 370 | - |
purified recombinant enzyme, Vmax | Thermotoga maritima |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified recombinant enzyme, 50 mM Tris-HCl, pH 7.5, stable | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-deoxy-D-glucose | 26% of the activity with D-glucose | Thermotoga maritima | ADP + 2-deoxy-D-glucose 6-phosphate | - |
ir | |
| ATP + D-glucose | the enzyme catalyzes the first step of the Embden-Meyerhoff pathway and the Entner-Douderoff pathway | Thermotoga maritima | ADP + D-glucose 6-phosphate | - |
ir | |
| ATP + D-glucose | the enzyme is specific for D-glucose | Thermotoga maritima | ADP + D-glucose 6-phosphate | - |
ir | |
| CTP + D-glucose | 5% of the activity with ATP as phosphoryl donor | Thermotoga maritima | CDP + D-glucose 6-phosphate | - |
? | |
| GTP + D-glucose | 12% of the activity with ATP as phosphoryl donor | Thermotoga maritima | GDP + D-glucose 6-phosphate | - |
? | |
| ITP + D-glucose | 20% of the activity with ATP as phosphoryl donor | Thermotoga maritima | IDP + D-glucose 6-phosphate | - |
? | |
| additional information | no activity with D-mannose and D-fructose as phosphoryl acceptors, no activity with UDP, ADP, acetyl phosphate, and diphosphate as phosphoryl donors | Thermotoga maritima | ? | - |
? | |
| UTP + D-glucose | 12% of the activity with ATP as phosphoryl donor | Thermotoga maritima | UDP + D-glucose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 36000, alpha2, recombinant enzyme, SDS-PAGE | Thermotoga maritima |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP-GLK | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 93 | - |
- |
Thermotoga maritima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | 98 | below 10% of maximal activity at 38°C, about 90% of maximal activity at 98°C | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
quite stable for 120 min | Thermotoga maritima |
| 100 | - |
almost complete inactivation after 5 min | Thermotoga maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Thermotoga maritima |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.7 | 50% activity at pH 6.8 and pH 7.7 | Thermotoga maritima |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Thermotoga maritima | |
| CTP | 5% of the activity with ATP | Thermotoga maritima | |
| GTP | 12% of the activity with ATP | Thermotoga maritima | |
| ITP | 20% of the activity with ATP | Thermotoga maritima | |
| UTP | 12% of the activity with ATP | Thermotoga maritima | |
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