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Literature summary for 2.7.1.2 extracted from

  • Meyer, D.; Schneider-Fresenius, C.; Horlacher, R.; Peist, R.; Boos, W.
    Molecular characterization of glucokinase from Escherichia coli K-12 (1997), J. Bacteriol., 179, 1298-1306.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.2

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli UE79 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.78
-
 D-glucose pH 7.65 Escherichia coli
3.76
-
 ATP pH 7.65 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, SDS-PAGE, overexpressed enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-glucose Escherichia coli
-
 ADP + D-glucose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 K-12
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
158
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
-
 Escherichia coli ADP + D-glucose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE, overexpressed enzyme Escherichia coli