General Stability | Organism |
---|---|
DTT stabilizes at 70°C | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Geobacillus stearothermophilus | |
AgNO3 | - |
Geobacillus stearothermophilus | |
D-glucose 6-phosphate | - |
Geobacillus stearothermophilus | |
D-maltose | - |
Geobacillus stearothermophilus | |
HgCl2 | - |
Geobacillus stearothermophilus | |
iodoacetamide | - |
Geobacillus stearothermophilus | |
N-acetyl-alpha-D-glucosamine | - |
Geobacillus stearothermophilus | |
N-ethylmaleimide | - |
Geobacillus stearothermophilus | |
p-chlormercuribenzoate | - |
Geobacillus stearothermophilus | |
p-hydroxymercuribenzoate | - |
Geobacillus stearothermophilus | |
Pb(NO3)2 | - |
Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
alpha-D-glucose | 30°C, pH 7.6 | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | no activation | Geobacillus stearothermophilus | |
Co2+ | 68% of the activity with Mg2+ | Geobacillus stearothermophilus | |
Mg2+ | required | Geobacillus stearothermophilus | |
Mn2+ | 45% of the activity with Mg2+ | Geobacillus stearothermophilus | |
Zn2+ | no activation | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | - |
HPLC gel filtration | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | Geobacillus stearothermophilus | - |
ADP + D-glucose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
large scale | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
334 | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose | - |
Geobacillus stearothermophilus | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | - |
Geobacillus stearothermophilus | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | highly specific for D-glucose | Geobacillus stearothermophilus | ADP + D-glucose 6-phosphate | - |
? | |
ATP + N-acetyl-alpha-D-glucosamine | at pH 7.6: 50% of the activity with alpha-D-glucose, at pH 9.0: 5% of the activity with alpha-D-glucose | Geobacillus stearothermophilus | ADP + N-acetyl-alpha-D-glucosamine 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | alpha2, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Geobacillus stearothermophilus |
60 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
DTT stabilizes at 70°C | Geobacillus stearothermophilus |
70 | - |
complete loss of activity in 70 min in presence of DTT | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10.5 | pH 6.0: about 30% of activity maximum, pH 10.5: about 80% of activity maximum | Geobacillus stearothermophilus |