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Literature summary for 2.7.1.2 extracted from
- Simultaneous purification and characterization of glucokinase, fructokinase and glucose-6-phosphate dehydrogenase from Zymomonas mobilis (1985), Biochem. J., 228, 627-634.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose 6-phosphate | weak | Zymomonas mobilis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
D-glucose | - |
Zymomonas mobilis | |
| 0.8 | - |
ATP | - |
Zymomonas mobilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | little or no activity with cations other than Mg2+ or Mn2+ | Zymomonas mobilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
alpha2, 2 * 33000, SDS-PAGE | Zymomonas mobilis |
| 65000 | - |
gel filtration | Zymomonas mobilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | Zymomonas mobilis | - |
ADP + D-glucose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zymomonas mobilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Zymomonas mobilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 240 | - |
- |
Zymomonas mobilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | - |
Zymomonas mobilis | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | highly specific for D-glucose | Zymomonas mobilis | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | phosphorylates alpha-D-glucose and beta-D-glucose equally fast | Zymomonas mobilis | ADP + D-glucose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | alpha2, 2 * 33000, SDS-PAGE | Zymomonas mobilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Zymomonas mobilis |
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Release 2023.1 (January 2023)
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