General Stability | Organism |
---|---|
rapid loss of activity in absence of DTT | Streptococcus mutans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-Amino-6-deoxy-D-glucose | noncompetitive to ATP and competitive to glucose | Streptococcus mutans | |
ADP | noncompetitive to ATP and uncompetitive to glucose | Streptococcus mutans | |
beta,gamma-methyleneadenosine 5'-thiophosphate | noncompetitive to ATP and uncompetitive to glucose | Streptococcus mutans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
ATP | - |
Streptococcus mutans | |
0.61 | - |
D-glucose | - |
Streptococcus mutans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 34% of the activation with Mg2+ | Streptococcus mutans | |
Co2+ | more efficient than Mg2+ in activation, relative rate 264% | Streptococcus mutans | |
Fe2+ | 48% of the activation with Mg2+ | Streptococcus mutans | |
Mg2+ | less efficient in activation than Mn2+ or Co2+ | Streptococcus mutans | |
Mn2+ | more efficient than Mg2+ in activation, relative rate 173% | Streptococcus mutans | |
Sr2+ | 11% of the activation with Mg2+ | Streptococcus mutans | |
Zn2+ | 56% of the activation with Mg2+ | Streptococcus mutans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | Streptococcus mutans | - |
ADP + D-glucose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | - |
OMZ70, ATCC 33535 | - |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus mutans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
198 | - |
- |
Streptococcus mutans |
Storage Stability | Organism |
---|---|
-20°C, 1 mM DTT, 20-30% glycerol, indefinitely stable | Streptococcus mutans |
room temperature, 1 mM DTT, 5 mM MgCl2, stable for 1 week , 4°C, suspended precipitate in a 60% saturated solution of ammonium sulfate, stable for at least 3 months | Streptococcus mutans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Streptococcus mutans | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | highly specific for D-glucose | Streptococcus mutans | ADP + D-glucose 6-phosphate | - |
? | |
CTP + D-glucose | less than 10% of the activity with ATP | Streptococcus mutans | CDP + D-glucose 6-phosphate | - |
? | |
GTP + D-glucose | less than 10% of ATP | Streptococcus mutans | GDP + D-glucose 6-phosphate | - |
? | |
ITP + D-glucose | less than 10% of the activity with ATP | Streptococcus mutans | IDP + D-glucose 6-phosphate | - |
? | |
additional information | less than 10% of the activity with ATP: TTP | Streptococcus mutans | ? | - |
? | |
UTP + D-glucose | less than 10% of the activity with ATP | Streptococcus mutans | UDP + D-glucose 6-phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | - |
Streptococcus mutans |