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Literature summary for 2.7.1.2 extracted from

  • Porter, V.; Chassy, B.M.
    Glucokinase from Streptococcus mutans (1982), Methods Enzymol., 90, 25-30.
No PubMed abstract available
Search for more literature for 2.7.1.2

General Stability

General Stability Organism
rapid loss of activity in absence of DTT Streptococcus mutans

Inhibitors

Inhibitors Comment Organism Structure
6-Amino-6-deoxy-D-glucose noncompetitive to ATP and competitive to glucose Streptococcus mutans
ADP noncompetitive to ATP and uncompetitive to glucose Streptococcus mutans
beta,gamma-methyleneadenosine 5'-thiophosphate noncompetitive to ATP and uncompetitive to glucose Streptococcus mutans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 ATP
-
 Streptococcus mutans
0.61
-
 D-glucose
-
 Streptococcus mutans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 34% of the activation with Mg2+ Streptococcus mutans
Co2+ more efficient than Mg2+ in activation, relative rate 264% Streptococcus mutans
Fe2+ 48% of the activation with Mg2+ Streptococcus mutans
Mg2+ less efficient in activation than Mn2+ or Co2+ Streptococcus mutans
Mn2+ more efficient than Mg2+ in activation, relative rate 173% Streptococcus mutans
Sr2+ 11% of the activation with Mg2+ Streptococcus mutans
Zn2+ 56% of the activation with Mg2+ Streptococcus mutans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-glucose Streptococcus mutans
-
 ADP + D-glucose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus mutans
-
 OMZ70, ATCC 33535
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus mutans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
198
-
-
 Streptococcus mutans

Storage Stability

Storage Stability Organism
-20°C, 1 mM DTT, 20-30% glycerol, indefinitely stable Streptococcus mutans
room temperature, 1 mM DTT, 5 mM MgCl2, stable for 1 week , 4°C, suspended precipitate in a 60% saturated solution of ammonium sulfate, stable for at least 3 months Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
-
 Streptococcus mutans ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose highly specific for D-glucose Streptococcus mutans ADP + D-glucose 6-phosphate
-
 ?
CTP + D-glucose less than 10% of the activity with ATP Streptococcus mutans CDP + D-glucose 6-phosphate
-
 ?
GTP + D-glucose less than 10% of ATP Streptococcus mutans GDP + D-glucose 6-phosphate
-
 ?
ITP + D-glucose less than 10% of the activity with ATP Streptococcus mutans IDP + D-glucose 6-phosphate
-
 ?
additional information less than 10% of the activity with ATP: TTP Streptococcus mutans ?
-
 ?
UTP + D-glucose less than 10% of the activity with ATP Streptococcus mutans UDP + D-glucose 6-phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9.5
-
 Streptococcus mutans