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Literature summary for 2.7.1.199 extracted from
- Staphylococcal phosphoenolpyruvate-dependent phosphotransferase system - two highly similar glucose permeases in Staphylococcus carnosus with different glucoside specificity: protein engineering in vivo? (1999), Microbiology, 145, 2881-2889.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Staphylococcus carnosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-deoxy-D-glucose | inhibitor of isoform EIICBAGlc1 | Staphylococcus carnosus |  |
| 2-Nitrophenyl beta-D-glucoside | inhibitor of isoform EIICBAGlc2 | Staphylococcus carnosus | |
| 4-nitrophenyl alpha-D-glucoside | inhibitor of isoform EIICBAGlc2 | Staphylococcus carnosus | |
| methyl alpha-D-glucoside | inhibitor of isoform EIICBAGlc2 | Staphylococcus carnosus | |
| methyl beta-D-glucoside | inhibitor of isoforms EIICBAGlc1 and EIICBAGlc2 | Staphylococcus carnosus | |
| Salicin | inhibitor of isoform EIICBAGlc2 | Staphylococcus carnosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
D-glucose[side 1] | membrane-bound isoform EIICBAGlc1, at pH 7.5 and 37°C | Staphylococcus carnosus | |
| 0.019 | - |
D-glucose[side 1] | membrane-bound isoform EIICBAGlc2, at pH 7.5 and 37°C | Staphylococcus carnosus | |
| 0.0305 | - |
D-glucose[side 1] | solubilized isoform EIICBAGlc1, at pH 7.5 and 37°C | Staphylococcus carnosus | |
| 0.0372 | - |
D-glucose[side 1] | solubilized isoform EIICBAGlc2, at pH 7.5 and 37°C | Staphylococcus carnosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Staphylococcus carnosus | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [protein]-Npi-phospho-L-histidine + D-glucose[side 1] | Staphylococcus carnosus | high specificity for D-glucose | [protein]-L-histidine + D-glucose 6-phosphate[side 2] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus carnosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-NTA column chromatography and Fractogel EMD DMAE Ion-exchange chromatography | Staphylococcus carnosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.125 | - |
solubilized isoform EIICBAGlc2 after 381fold purification, at pH 7.5 and 37°C | Staphylococcus carnosus |
| 6.019 | - |
solubilized isoform EIICBAGlc1 after 255fold purification, at pH 7.5 and 37°C | Staphylococcus carnosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | natural mono- or disaccharides, such as mannose or N-acetylglucosamine are not phosphorylated by either isoform EIICBAGlc1 nor EIICBAGlc2 | Staphylococcus carnosus | ? | - |
? | |
| [protein]-Npi-phospho-L-histidine + D-glucose[side 1] | high specificity for D-glucose | Staphylococcus carnosus | [protein]-L-histidine + D-glucose 6-phosphate[side 2] | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EIICBAGlc1 | isoform | Staphylococcus carnosus |
| EIICBAGlc2 | isoform | Staphylococcus carnosus |
| glcA | - |
Staphylococcus carnosus |
| glucose permease | - |
Staphylococcus carnosus |
| PtsG | - |
Staphylococcus carnosus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0094 | - |
methyl beta-D-glucoside | solubilized isoform EIICBAGlc1, at pH 7.5 and 37°C | Staphylococcus carnosus | |
| 0.0179 | - |
Salicin | solubilized isoform EIICBAGlc2, at pH 7.5 and 37°C | Staphylococcus carnosus | |
| 0.0218 | - |
methyl beta-D-glucoside | solubilized isoform EIICBAGlc2, at pH 7.5 and 37°C | Staphylococcus carnosus | |
| 0.0285 | - |
2-Nitrophenyl beta-D-glucoside | solubilized isoform EIICBAGlc2, at pH 7.5 and 37°C | Staphylococcus carnosus | |
| 0.0321 | - |
4-nitrophenyl alpha-D-glucoside | solubilized isoform EIICBAGlc2, at pH 7.5 and 37°C | Staphylococcus carnosus | |
| 0.0349 | - |
methyl alpha-D-glucoside | solubilized isoform EIICBAGlc2, at pH 7.5 and 37°C | Staphylococcus carnosus | |
| 0.0397 | - |
2-deoxy-D-glucose | solubilized isoform EIICBAGlc1, at pH 7.5 and 37°C | Staphylococcus carnosus | |
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