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Literature summary for 2.7.1.197 extracted from

  • Weng, Q.; Elder, J.; Jacobson, G.
    Site-specific mutagenesis of residues in the Escherichia coli mannitol permease that have been suggested to be important for its phosphorylation and chemoreception functions (1992), J. Biol. Chem., 267, 19529-19535.
    View publication on PubMed
Search for more literature for 2.7.1.197

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli LGS322 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
C384D inactive Escherichia coli
C384H inactive Escherichia coli
H156A the mutant shows wild type activity Escherichia coli
H195A the mutant shows very low activity compared to the wild type enzyme Escherichia coli
H195N the mutant shows 30% of wild type activity Escherichia coli
H195R the mutant shows very low activity compared to the wild type enzyme Escherichia coli
H554A inactive Escherichia coli
H554D inactive Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[protein]-Npi-phospho-L-histidine + D-mannitol[side 1] Escherichia coli
-
 [protein]-L-histidine + D-mannitol 1-phosphate[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P00550
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[protein]-Npi-phospho-L-histidine + D-mannitol[side 1]
-
 Escherichia coli [protein]-L-histidine + D-mannitol 1-phosphate[side 2]
-
 ?

Synonyms

Synonyms Comment Organism
EIIMtl
-
 Escherichia coli
mannitol permease
-
 Escherichia coli
mtlA
-
 Escherichia coli