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Literature summary for 2.7.1.190 extracted from

  • Shi, K.; Berghuis, A.M.
    Structural basis for dual nucleotide selectivity of aminoglycoside 2-phosphotransferase IVa provides insight on determinants of nucleotide specificity of aminoglycoside kinases (2012), J. Biol. Chem., 287, 13094-13102.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.190

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of isoform APH(2'')-IVa, wild-type and mutant F95M, in complex with either adenosine or guanosine Enterococcus casseliflavus

Protein Variants

Protein Variants Comment Organism
F95M no significantly different binding affinities as compared with the wild-type Enterococcus casseliflavus
F95Y mutation shifts the nucleotide selectivity from a 2.5fold preference for ATP to a 1.5fold preference for GTP Enterococcus casseliflavus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.049
-
 GTP mutant F95Y, pH 7.5, 37°C Enterococcus casseliflavus
0.055
-
 ATP mutant F95M, pH 7.5, 37°C Enterococcus casseliflavus
0.069
-
 ATP wild-type, pH 7.5, 37°C Enterococcus casseliflavus
0.072
-
 ATP mutant F95Y, pH 7.5, 37°C Enterococcus casseliflavus
0.156
-
 GTP mutant F95M, pH 7.5, 37°C Enterococcus casseliflavus
0.168
-
 GTP wild-type, pH 7.5, 37°C Enterococcus casseliflavus

Organism

Organism UniProt Comment Textmining
Enterococcus casseliflavus O68183
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + tobramycin
-
 Enterococcus casseliflavus ADP + tobramycin 2''-phosphate
-
 ?
GTP + tobramycin
-
 Enterococcus casseliflavus GDP + tobramycin 2''-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
APH(2'')-Id
-
 Enterococcus casseliflavus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.06
-
 ATP mutant F95Y, pH 7.5, 37°C Enterococcus casseliflavus
1.15
-
 GTP mutant F95Y, pH 7.5, 37°C Enterococcus casseliflavus
3.06
-
 ATP mutant F95M, pH 7.5, 37°C Enterococcus casseliflavus
4.35
-
 GTP mutant F95M, pH 7.5, 37°C Enterococcus casseliflavus
4.37
-
 ATP wild-type, pH 7.5, 37°C Enterococcus casseliflavus
5.03
-
 GTP wild-type, pH 7.5, 37°C Enterococcus casseliflavus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15
-
 ATP mutant F95Y, pH 7.5, 37°C Enterococcus casseliflavus
23
-
 GTP mutant F95Y, pH 7.5, 37°C Enterococcus casseliflavus
28
-
 GTP mutant F95M, pH 7.5, 37°C Enterococcus casseliflavus
30
-
 GTP wild-type, pH 7.5, 37°C Enterococcus casseliflavus
55
-
 ATP mutant F95M, pH 7.5, 37°C Enterococcus casseliflavus
63
-
 ATP wild-type, pH 7.5, 37°C Enterococcus casseliflavus