Inhibitors | Comment | Organism | Structure |
---|---|---|---|
apramycin | - |
Enterococcus sp. | |
butirosin | - |
Enterococcus sp. | |
lividomycin A | - |
Enterococcus sp. | |
neamine | - |
Enterococcus sp. | |
neomycin | - |
Enterococcus sp. | |
paromomycin | - |
Enterococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
kanamycin A | pH 7.5, 25°C | Enterococcus sp. | |
0.0045 | - |
GTP | pH 7.5, 25°C, cosubstrate kanamycin A | Enterococcus sp. | |
0.039 | - |
ITP | pH 7.5, 25°C, cosubstrate kanamycin A | Enterococcus sp. | |
0.09 | - |
ATP | pH 7.5, 25°C, cosubstrate kanamycin A | Enterococcus sp. | |
0.1 | - |
UTP | pH 7.5, 25°C, cosubstrate kanamycin A | Enterococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + kanamycin A | - |
Enterococcus sp. | ADP + kanamycin A 2''-phosphate | - |
? | |
GTP + gentamicin C | - |
Enterococcus sp. | GDP + gentamicin C 2''-phosphate | GTP is preferred over ATP | ? | |
GTP + netilmicin | - |
Enterococcus sp. | GDP + netilmicin 2''-phosphate | - |
? | |
ITP + dibekacin | - |
Enterococcus sp. | IDP + dibekacin 2''-phosphate | - |
? | |
additional information | random sequential Bi Bi mechanism. At pH 7.5 the release of guanosine triphosphate is rate-limiting. No substrates: amikacin, isepamicin, neomycin, butirosin, lividomycin A , paromomycin, spectinomycin, streptomycin, apramycin, hygromycin, neamine | Enterococcus sp. | ? | - |
? | |
UTP + tobramycin | - |
Enterococcus sp. | UDP + tobramycin 2''-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aminoglycoside 2''-phosphotransferase type IIIa | - |
Enterococcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
ATP | pH 7.5, 25°C, cosubstrate kanamycin A | Enterococcus sp. | |
0.14 | - |
UTP | pH 7.5, 25°C, cosubstrate kanamycin A | Enterococcus sp. | |
0.5 | - |
gentamicin C | pH 7.5, 25°C | Enterococcus sp. | |
0.7 | - |
tobramycin | pH 7.5, 25°C | Enterococcus sp. | |
0.7 | - |
netilmicin | pH 7.5, 25°C | Enterococcus sp. | |
0.7 | - |
dibekacin | pH 7.5, 25°C | Enterococcus sp. | |
0.8 | - |
kanamycin A | pH 7.5, 25°C | Enterococcus sp. | |
0.8 | - |
GTP | pH 7.5, 25°C, cosubstrate kanamycin A | Enterococcus sp. | |
2.1 | - |
ITP | pH 7.5, 25°C, cosubstrate kanamycin A | Enterococcus sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
ATP | pH 7.5, 25°C, cosubstrate kanamycin A | Enterococcus sp. | |
1.4 | - |
UTP | pH 7.5, 25°C, cosubstrate kanamycin A | Enterococcus sp. | |
56 | - |
ITP | pH 7.5, 25°C, cosubstrate kanamycin A | Enterococcus sp. | |
180 | - |
kanamycin A | pH 7.5, 25°C | Enterococcus sp. | |
180 | - |
GTP | pH 7.5, 25°C, cosubstrate kanamycin A | Enterococcus sp. | |
670 | - |
netilmicin | pH 7.5, 25°C | Enterococcus sp. | |
1800 | - |
gentamicin C | pH 7.5, 25°C | Enterococcus sp. | |
2200 | - |
tobramycin | pH 7.5, 25°C | Enterococcus sp. | |
2300 | - |
dibekacin | pH 7.5, 25°C | Enterococcus sp. |