BRENDA - Enzyme Database
show all sequences of 2.7.1.190

Novel aminoglycoside 2-phosphotransferase identified in a gram-negative pathogen

Toth, M.; Frase, H.; Antunes, N.T.; Vakulenko, S.B.; Antimicrob. Agents Chemother. 57, 452-457 (2013)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
lividomycin A
-
Campylobacter jejuni
neamine
-
Campylobacter jejuni
neomycin B
-
Campylobacter jejuni
paromomycin
-
Campylobacter jejuni
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00055
-
kanamycin B
pH 7.0, 22°C
Campylobacter jejuni
0.0007
-
gentamicin C
pH 7.0, 22°C
Campylobacter jejuni
0.0008
-
GTP
pH 7.0, 22°C
Campylobacter jejuni
0.001
-
tobramycin
above 0.001, pH 7.0, 22°C
Campylobacter jejuni
0.0011
-
kanamycin A
pH 7.0, 22°C
Campylobacter jejuni
0.0012
-
dibekacin
pH 7.0, 22°C
Campylobacter jejuni
0.0016
-
sisomicin
pH 7.0, 22°C
Campylobacter jejuni
0.0062
-
arbekacin
pH 7.0, 22°C
Campylobacter jejuni
0.01
-
netilmicin
pH 7.0, 22°C
Campylobacter jejuni
0.26
-
amikacin
pH 7.0, 22°C
Campylobacter jejuni
0.56
-
isepamicin
pH 7.0, 22°C
Campylobacter jejuni
2
-
ATP
pH 7.0, 22°C
Campylobacter jejuni
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Campylobacter jejuni
Q4VR94
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + gentamicin
-
733113
Campylobacter jejuni
ADP + gentamicin 2''-phosphate
GTP is preferred over ATP
-
-
?
GTP + amikacin
-
733113
Campylobacter jejuni
GDP + amikacin 2''-phosphate
-
-
-
?
GTP + arbekacin
-
733113
Campylobacter jejuni
GDP + arbekacin 2''-phosphate
-
-
-
?
GTP + dibekacin
-
733113
Campylobacter jejuni
GDP + dibekacin 2''-phosphate
-
-
-
?
GTP + gentamicin C
-
733113
Campylobacter jejuni
GDP + gentamicin C 2''-phosphate
GTP is preferred over ATP
-
-
?
GTP + isepamicin
-
733113
Campylobacter jejuni
GDP + isepamicin 2''-phosphate
-
-
-
?
GTP + kanamycin A
-
733113
Campylobacter jejuni
GDP + kanamycin A 2''-phosphate
-
-
-
?
GTP + kanamycin B
-
733113
Campylobacter jejuni
GDP + kanamycin B 2''-phosphate
-
-
-
?
GTP + netilmicin
-
733113
Campylobacter jejuni
GDP + netilmicin 2''-phosphate
-
-
-
?
GTP + sisomicin
-
733113
Campylobacter jejuni
GDP + sisomicin 2''-phosphate
-
-
-
?
GTP + tobramycin
-
733113
Campylobacter jejuni
GDP + tobramycin 2''-phosphate
-
-
-
?
additional information
no substrates: 4,5-disubstituted antibiotics and the atypical aminoglycoside neamine
733113
Campylobacter jejuni
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.25
-
isepamicin
pH 7.0, 22°C
Campylobacter jejuni
0.38
-
ATP
pH 7.0, 22°C
Campylobacter jejuni
0.38
-
kanamycin B
pH 7.0, 22°C
Campylobacter jejuni
0.4
-
GTP
pH 7.0, 22°C
Campylobacter jejuni
0.48
-
tobramycin
pH 7.0, 22°C
Campylobacter jejuni
0.79
-
dibekacin
pH 7.0, 22°C
Campylobacter jejuni
1.7
-
kanamycin A
pH 7.0, 22°C
Campylobacter jejuni
4.3
-
arbekacin
pH 7.0, 22°C
Campylobacter jejuni
6.5
-
amikacin
pH 7.0, 22°C
Campylobacter jejuni
8.3
-
gentamicin C
pH 7.0, 22°C
Campylobacter jejuni
17
-
sisomicin
pH 7.0, 22°C
Campylobacter jejuni
18
-
netilmicin
pH 7.0, 22°C
Campylobacter jejuni
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.00016
-
neamine
pH 7.0, 22°C
Campylobacter jejuni
0.00073
-
neomycin B
pH 7.0, 22°C
Campylobacter jejuni
0.0085
-
paromomycin
pH 7.0, 22°C
Campylobacter jejuni
0.012
-
lividomycin A
pH 7.0, 22°C
Campylobacter jejuni
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
lividomycin A
-
Campylobacter jejuni
neamine
-
Campylobacter jejuni
neomycin B
-
Campylobacter jejuni
paromomycin
-
Campylobacter jejuni
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.00016
-
neamine
pH 7.0, 22°C
Campylobacter jejuni
0.00073
-
neomycin B
pH 7.0, 22°C
Campylobacter jejuni
0.0085
-
paromomycin
pH 7.0, 22°C
Campylobacter jejuni
0.012
-
lividomycin A
pH 7.0, 22°C
Campylobacter jejuni
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00055
-
kanamycin B
pH 7.0, 22°C
Campylobacter jejuni
0.0007
-
gentamicin C
pH 7.0, 22°C
Campylobacter jejuni
0.0008
-
GTP
pH 7.0, 22°C
Campylobacter jejuni
0.001
-
tobramycin
above 0.001, pH 7.0, 22°C
Campylobacter jejuni
0.0011
-
kanamycin A
pH 7.0, 22°C
Campylobacter jejuni
0.0012
-
dibekacin
pH 7.0, 22°C
Campylobacter jejuni
0.0016
-
sisomicin
pH 7.0, 22°C
Campylobacter jejuni
0.0062
-
arbekacin
pH 7.0, 22°C
Campylobacter jejuni
0.01
-
netilmicin
pH 7.0, 22°C
Campylobacter jejuni
0.26
-
amikacin
pH 7.0, 22°C
Campylobacter jejuni
0.56
-
isepamicin
pH 7.0, 22°C
Campylobacter jejuni
2
-
ATP
pH 7.0, 22°C
Campylobacter jejuni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + gentamicin
-
733113
Campylobacter jejuni
ADP + gentamicin 2''-phosphate
GTP is preferred over ATP
-
-
?
GTP + amikacin
-
733113
Campylobacter jejuni
GDP + amikacin 2''-phosphate
-
-
-
?
GTP + arbekacin
-
733113
Campylobacter jejuni
GDP + arbekacin 2''-phosphate
-
-
-
?
GTP + dibekacin
-
733113
Campylobacter jejuni
GDP + dibekacin 2''-phosphate
-
-
-
?
GTP + gentamicin C
-
733113
Campylobacter jejuni
GDP + gentamicin C 2''-phosphate
GTP is preferred over ATP
-
-
?
GTP + isepamicin
-
733113
Campylobacter jejuni
GDP + isepamicin 2''-phosphate
-
-
-
?
GTP + kanamycin A
-
733113
Campylobacter jejuni
GDP + kanamycin A 2''-phosphate
-
-
-
?
GTP + kanamycin B
-
733113
Campylobacter jejuni
GDP + kanamycin B 2''-phosphate
-
-
-
?
GTP + netilmicin
-
733113
Campylobacter jejuni
GDP + netilmicin 2''-phosphate
-
-
-
?
GTP + sisomicin
-
733113
Campylobacter jejuni
GDP + sisomicin 2''-phosphate
-
-
-
?
GTP + tobramycin
-
733113
Campylobacter jejuni
GDP + tobramycin 2''-phosphate
-
-
-
?
additional information
no substrates: 4,5-disubstituted antibiotics and the atypical aminoglycoside neamine
733113
Campylobacter jejuni
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.25
-
isepamicin
pH 7.0, 22°C
Campylobacter jejuni
0.38
-
ATP
pH 7.0, 22°C
Campylobacter jejuni
0.38
-
kanamycin B
pH 7.0, 22°C
Campylobacter jejuni
0.4
-
GTP
pH 7.0, 22°C
Campylobacter jejuni
0.48
-
tobramycin
pH 7.0, 22°C
Campylobacter jejuni
0.79
-
dibekacin
pH 7.0, 22°C
Campylobacter jejuni
1.7
-
kanamycin A
pH 7.0, 22°C
Campylobacter jejuni
4.3
-
arbekacin
pH 7.0, 22°C
Campylobacter jejuni
6.5
-
amikacin
pH 7.0, 22°C
Campylobacter jejuni
8.3
-
gentamicin C
pH 7.0, 22°C
Campylobacter jejuni
17
-
sisomicin
pH 7.0, 22°C
Campylobacter jejuni
18
-
netilmicin
pH 7.0, 22°C
Campylobacter jejuni
General Information
General Information
Commentary
Organism
physiological function
expression in Escherichia coli confers resistance to the 4,6-disubstituted aminoglycosides kanamycin, tobramycin, dibekacin, gentamicin, and sisomicin, but not to arbekacin, amikacin, isepamicin, or netilmicin, but not to any of the 4,5-disubstituted antibiotics tested
Campylobacter jejuni
General Information (protein specific)
General Information
Commentary
Organism
physiological function
expression in Escherichia coli confers resistance to the 4,6-disubstituted aminoglycosides kanamycin, tobramycin, dibekacin, gentamicin, and sisomicin, but not to arbekacin, amikacin, isepamicin, or netilmicin, but not to any of the 4,5-disubstituted antibiotics tested
Campylobacter jejuni
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.19
-
ATP
pH 7.0, 22°C
Campylobacter jejuni
0.45
-
isepamicin
pH 7.0, 22°C
Campylobacter jejuni
25
-
amikacin
pH 7.0, 22°C
Campylobacter jejuni
480
-
tobramycin
above 480, pH 7.0, 22°C
Campylobacter jejuni
510
-
GTP
pH 7.0, 22°C
Campylobacter jejuni
660
-
dibekacin
pH 7.0, 22°C
Campylobacter jejuni
690
-
arbekacin
pH 7.0, 22°C
Campylobacter jejuni
690
-
kanamycin B
pH 7.0, 22°C
Campylobacter jejuni
1500
-
kanamycin A
pH 7.0, 22°C
Campylobacter jejuni
1700
-
netilmicin
pH 7.0, 22°C
Campylobacter jejuni
11000
-
sisomicin
pH 7.0, 22°C
Campylobacter jejuni
12000
-
gentamicin C
pH 7.0, 22°C
Campylobacter jejuni
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.19
-
ATP
pH 7.0, 22°C
Campylobacter jejuni
0.45
-
isepamicin
pH 7.0, 22°C
Campylobacter jejuni
25
-
amikacin
pH 7.0, 22°C
Campylobacter jejuni
480
-
tobramycin
above 480, pH 7.0, 22°C
Campylobacter jejuni
510
-
GTP
pH 7.0, 22°C
Campylobacter jejuni
660
-
dibekacin
pH 7.0, 22°C
Campylobacter jejuni
690
-
arbekacin
pH 7.0, 22°C
Campylobacter jejuni
690
-
kanamycin B
pH 7.0, 22°C
Campylobacter jejuni
1500
-
kanamycin A
pH 7.0, 22°C
Campylobacter jejuni
1700
-
netilmicin
pH 7.0, 22°C
Campylobacter jejuni
11000
-
sisomicin
pH 7.0, 22°C
Campylobacter jejuni
12000
-
gentamicin C
pH 7.0, 22°C
Campylobacter jejuni
Other publictions for EC 2.7.1.190
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733113
Toth
Novel aminoglycoside 2-phospho ...
Campylobacter jejuni
Antimicrob. Agents Chemother.
57
452-457
2013
-
-
-
-
-
-
4
12
-
-
-
-
-
2
-
-
-
-
-
-
-
-
12
-
-
-
-
12
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
4
4
12
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
12
-
-
-
-
-
1
1
-
12
12
734173
Smith
Aminoglycoside 2-phosphotransf ...
Enterococcus gallinarum
J. Biol. Chem.
287
12893-12903
2012
-
-
1
1
1
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734174
Shi
Structural basis for dual nucl ...
Enterococcus casseliflavus
J. Biol. Chem.
287
13094-13102
2012
-
-
-
1
2
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
734204
Frase
Revisiting the nucleotide and ...
Staphylococcus aureus
J. Biol. Chem.
287
43262-43269
2012
-
-
-
-
-
-
4
6
-
-
-
1
-
1
-
-
-
-
-
-
-
-
10
-
-
-
-
9
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
4
4
6
-
-
-
1
-
-
-
-
-
-
-
-
10
-
-
-
-
9
-
-
-
-
-
1
1
-
6
6
733329
Shi
Crystal structures of antibiot ...
Enterococcus casseliflavus
Biochemistry
50
6237-6244
2011
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734160
Badarau
Aminoglycoside 2''-phosphotran ...
Enterococcus sp.
J. Biol. Chem.
283
7638-7647
2008
-
-
-
-
-
-
6
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
5
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
9
-
-
-
-
-
-
-
-
9
9
734051
Ferretti
Nucleotide sequence analysis o ...
Enterococcus faecalis, Enterococcus faecalis ATCC 700802
J. Bacteriol.
167
631-638
1986
-
-
1
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-